pan 14-3-3 Antibody (B-11): sc-133232

pan 14-3-3 Antibody (B-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects pan 14-3-3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). pan 14-3-3 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 14-3-3 proteins play a crucial role in regulating numerous cellular processes, particularly those associated with cancer biology, such as apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 proteins are located primarily in the cytoplasm and nucleus, where 14-3-3 proteins interact with a wide range of target proteins, influencing their activity and stability. 14-3-3 proteins′ ability to sequester ligands in the cytoplasm is particularly important, as this sequestration prevents ligand degradation and modulates interactions with other cellular components. Such dynamic regulation is essential for maintaining cellular homeostasis and ensuring proper cellular responses to various stimuli. Loss of 14-3-3 expression can lead to dysregulation of these processes, contributing to the development of various diseases, including cancer.

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pan 14-3-3 Antibody (B-11) References:

1. Specific interactions with TBP and TFIIB in vitro suggest that 14-3-3 proteins may participate in the regulation of transcription when part of a DNA binding complex.  |  Pan, S., et al. 1999. Plant Cell. 11: 1591-602. PMID: 10449590
2. Multiple elements regulate nuclear/cytoplasmic shuttling of FOXO1: characterization of phosphorylation- and 14-3-3-dependent and -independent mechanisms.  |  Zhao, X., et al. 2004. Biochem J. 378: 839-49. PMID: 14664696
3. [14-3-3 proteins--a role in the regulation of melatonin biosynthesis].  |  Rosiak, J. and Zawilska, JB. 2006. Postepy Biochem. 52: 35-41. PMID: 16869299
4. The value of serial cerebrospinal fluid 14-3-3 protein levels in adult community-acquired bacterial meningitis.  |  Lu, CH., et al. 2008. QJM. 101: 225-30. PMID: 18234734
5. Functional identification of a novel 14-3-3 epsilon splicing variant suggests dimerization is not necessary for 14-3-3 epsilon to inhibit UV-induced apoptosis.  |  Han, D., et al. 2010. Biochem Biophys Res Commun. 396: 401-6. PMID: 20417184
6. Phosphorylation-dependent 14-3-3 binding to LRRK2 is impaired by common mutations of familial Parkinson's disease.  |  Li, X., et al. 2011. PLoS One. 6: e17153. PMID: 21390248
7. Colocalization of 14-3-3 proteins with SOD1 in Lewy body-like hyaline inclusions in familial amyotrophic lateral sclerosis cases and the animal model.  |  Okamoto, Y., et al. 2011. PLoS One. 6: e20427. PMID: 21655264
8. Roles of vimentin and 14-3-3 zeta/delta in the inhibitory effects of heparin on PC-3M cell proliferation and B16-F10-luc-G5 cells metastasis.  |  Pan, Y., et al. 2012. Acta Pharmacol Sin. 33: 798-808. PMID: 22669117
9. Discovery of 14-3-3 protein-protein interaction inhibitors that sensitize multidrug-resistant cancer cells to doxorubicin and the Akt inhibitor GSK690693.  |  Mori, M., et al. 2014. ChemMedChem. 9: 973-83. PMID: 24715717
10. Ectopic over-expression of HaFT-1, a 14-3-3 protein from Haloxylon ammodendron, enhances acquired thermotolerance in transgenic Arabidopsis.  |  Pan, R., et al. 2023. Plant Mol Biol. 112: 261-277. PMID: 37341869

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan 14-3-3 Antibody (B-11) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-pan 14-3-3 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan 14-3-3 Antibody (B-11) Specifications

Alternate Product Name: 14-3-3

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-246

Epitope Target: 14-3-3 β

Epitope Species: human

Product Reactivity/Detection: pan 14-3-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for pan 14-3-3 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred pan 14-3-3 antibody is pan 14-3-3 Antibody (H-8): sc-1657.