Paip1 Antibody (E-7): sc-365687
- Paip1 Antibody (E-7) is a mouse monoclonal IgG2b κ Paip1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 180-479 mapping at the C-terminus of Paip1 of human origin
- Paip1 Antibody (E-7) is recommended for detection of Paip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Paip1 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Paip1 Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with Paip1 Antibody (E-7) (see ordering information below).
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Paip1 Antibody (E-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Paip1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Paip1 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Paip1, known as PABP-interacting protein, plays a crucial role in translation regulation by binding to polyadenylate-binding protein (PABP). This interaction enhances mRNA stability and translation efficiency, which is vital for proper gene expression and cellular function. Paip1 competes with Paip2 for binding to PABP; while Paip1 stimulates translation, Paip2 acts as a repressor by decreasing PABP′s affinity for polyadenylate RNA. The human Paip1 gene encodes a 480 amino acid protein featuring an eIF4A-binding region and a proline-rich N terminus, which are essential for linking mRNA termini and facilitating translation initiation. Paip1 overexpression in COS-7 cells stimulates translation, highlighting its importance in protein synthesis regulation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Paip1 Antibody (E-7) References:
- Multiple portions of poly(A)-binding protein stimulate translation in vivo. | Gray, NK., et al. 2000. EMBO J. 19: 4723-33. PMID: 10970864
- A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. | Grosset, C., et al. 2000. Cell. 103: 29-40. PMID: 11051545
- Structure and function of the C-terminal PABC domain of human poly(A)-binding protein. | Kozlov, G., et al. 2001. Proc Natl Acad Sci U S A. 98: 4409-13. PMID: 11287632
- Dual interactions of the translational repressor Paip2 with poly(A) binding protein. | Khaleghpour, K., et al. 2001. Mol Cell Biol. 21: 5200-13. PMID: 11438674
- Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2. | Gouyon, F., et al. 2003. Biochem J. 375: 167-74. PMID: 12820898
- Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation. | Craig, AW., et al. 1998. Nature. 392: 520-3. PMID: 9548260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Paip1 Antibody (E-7) | sc-365687 | 200 µg/ml | $316.00 | |||
Paip1 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-522330 | 200 µg Ab, 40 µg BP | $354.00 | |||
Paip1 Antibody (E-7): m-IgG2b BP-HRP Bundle | sc-549021 | 200 µg Ab; 10 µg BP | $354.00 | |||
Paip1 Antibody (E-7) AC | sc-365687 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Paip1 Antibody (E-7) HRP | sc-365687 HRP | 200 µg/ml | $316.00 | |||
Paip1 Antibody (E-7) FITC | sc-365687 FITC | 200 µg/ml | $330.00 | |||
Paip1 Antibody (E-7) PE | sc-365687 PE | 200 µg/ml | $343.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 488 | sc-365687 AF488 | 200 µg/ml | $357.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 546 | sc-365687 AF546 | 200 µg/ml | $357.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 594 | sc-365687 AF594 | 200 µg/ml | $357.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 647 | sc-365687 AF647 | 200 µg/ml | $357.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 680 | sc-365687 AF680 | 200 µg/ml | $357.00 | |||
Paip1 Antibody (E-7) Alexa Fluor® 790 | sc-365687 AF790 | 200 µg/ml | $357.00 |