Paip1 Activators
Chemical activators of Paip1 can influence its role in translation initiation through various intracellular pathways and stress responses. Leptomycin B, by inhibiting Exportin 1 (CRM1), leads to the nuclear retention of RNA-binding proteins, which may enhance the interaction of Paip1 with elements of the translation machinery, thus facilitating its activation. Similarly, MG132, a proteasome inhibitor, prevents the degradation of proteins involved in mRNA translation. This stabilization can lead to an increase in protein synthesis, indirectly enhancing the activity of Paip1 in the formation of translation initiation complexes. Okadaic Acid acts by inhibiting protein phosphatases such as PP1 and PP2A, leading to sustained phosphorylation states of proteins, which could bolster the activity of Paip1 by promoting its association with phosphorylated translation initiation factors.
On the other hand, compounds like Sodium Arsenite induce the formation of stress granules, where Paip1's role may be amplified as part of the cellular stress response, thus promoting its functional activity in mRNA stabilization and translation. Cycloheximide and Anisomycin disrupt translation elongation and peptide bond formation, respectively, potentially causing an accumulation of translation initiation complexes, where Paip1 could play a more prominent role. Rapamycin, by inhibiting mTORC1, can lead to an upregulation of the translation initiation phase, thereby enhancing the functional activity of Paip1. Puromycin, which causes premature chain termination, and Emetine, which blocks ribosomal translocation, can both lead to an increased demand and formation of translation initiation complexes, indirectly promoting Paip1 activity. Patulin induces ribotoxic stress, possibly leading to an upregulation of translation initiation mechanisms, while Homoharringtonine, by inhibiting initial elongation, may also indirectly enhance Paip1's role in translation initiation. Lastly, Chloroquine's inhibition of lysosomal enzymes and alteration of endosomal pH can trigger cellular stress responses that may enhance the translational processes where Paip1 is critically involved, indirectly increasing its functional activity.
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| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Leptomycin B | 87081-35-4 | sc-358688 sc-358688A sc-358688B | 50 µg 500 µg 2.5 mg | $105.00 $408.00 $1224.00 | 35 | |
Leptomycin B inhibits the nuclear export receptor Exportin 1 (CRM1). The inhibition of CRM1 can lead to the nuclear accumulation of RNA-binding proteins, which may enhance the functional activity of Paip1 by promoting its interaction with translation initiation factors and mRNA in the nucleus. | ||||||
MG-132 [Z-Leu- Leu-Leu-CHO] | 133407-82-6 | sc-201270 sc-201270A sc-201270B | 5 mg 25 mg 100 mg | $56.00 $260.00 $980.00 | 163 | |
MG132 is a proteasome inhibitor that can prevent the degradation of proteins, including those involved in mRNA translation. Stabilization of these proteins can lead to increased protein synthesis, which may indirectly enhance the function of Paip1 in the translation initiation complex. | ||||||
Okadaic Acid | 78111-17-8 | sc-3513 sc-3513A sc-3513B | 25 µg 100 µg 1 mg | $285.00 $520.00 $1300.00 | 78 | |
Okadaic Acid is a potent inhibitor of protein phosphatases PP1 and PP2A. Inhibition of these phosphatases can lead to sustained phosphorylation of translation factors, which could enhance the functional interaction of Paip1 with the translation initiation machinery. | ||||||
Sodium (meta)arsenite | 7784-46-5 | sc-250986 sc-250986A | 100 g 1 kg | $106.00 $765.00 | 3 | |
Sodium Arsenite induces stress granule formation. Paip1, being associated with mRNA regulation, could be functionally activated as a part of the stress response, potentially enhancing its role in mRNA stabilization and translation under stress conditions. | ||||||
Cycloheximide | 66-81-9 | sc-3508B sc-3508 sc-3508A | 100 mg 1 g 5 g | $40.00 $82.00 $256.00 | 127 | |
Cycloheximide inhibits eukaryotic protein synthesis by interfering with the translocation step. This can cause an accumulation of translation initiation complexes, which may indirectly increase the functional activity of Paip1 as it is involved in the initiation of translation. | ||||||
Anisomycin | 22862-76-6 | sc-3524 sc-3524A | 5 mg 50 mg | $97.00 $254.00 | 36 | |
Anisomycin disrupts peptide bond formation, which can lead to an accumulation of translation initiation complexes. This build-up can potentially enhance the role of Paip1 in the initiation of translation by increasing its interaction with the translation machinery. | ||||||
Puromycin | 53-79-2 | sc-205821 sc-205821A | 10 mg 25 mg | $163.00 $316.00 | 436 | |
Puromycin causes premature chain termination during protein synthesis. The interference in translation may lead to an increased demand for functional translation initiation, potentially enhancing the activity of Paip1 in the formation of the translation initiation complex. | ||||||
Emetine | 483-18-1 | sc-470668 sc-470668A sc-470668B sc-470668C | 1 mg 10 mg 50 mg 100 mg | $352.00 $566.00 $1331.00 $2453.00 | ||
Emetine inhibits protein synthesis by blocking the movement of ribosomes along mRNA. The inhibition at this stage can lead to a compensatory increase in translation initiation, potentially enhancing the activity of Paip1. | ||||||
Homoharringtonine | 26833-87-4 | sc-202652 sc-202652A sc-202652B | 1 mg 5 mg 10 mg | $51.00 $123.00 $178.00 | 11 | |
Homoharringtonine inhibits protein synthesis by preventing the initial elongation step on the 80S ribosome. This may result in increased initiation complex formation, indirectly enhancing the functional activity of Paip1 in translation initiation. | ||||||
Chloroquine | 54-05-7 | sc-507304 | 250 mg | $68.00 | 2 | |
Chloroquine inhibits lysosomal enzymes and raises endosomal pH. This can lead to a general increase in cellular stress and a compensatory upregulation of protein synthesis mechanisms, indirectly enhancing the functional activity of Paip1 in translation initiation. | ||||||