PACAP-related peptide Antibody (D-4): sc-365356

PACAP-related peptide Antibody (D-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects PACAP-related peptide of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PACAP-related peptide (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PACAP-related peptide, also known as adenylate cyclase activating polypeptide 1, plays a crucial role in the neuroendocrine stress response and is part of a superfamily of peptide hormones that includes vasoactive intestinal peptide. PACAP-related peptide is primarily located in the central nervous system and peripheral tissues, where PACAP-related peptide is involved in various physiological processes, including neuronal differentiation modulation and immune response regulation. PACAP-related peptide localization in the brain is particularly significant, as PACAP-related peptide is implicated in neuroprotection, neurogenesis, and synaptic plasticity modulation, which are essential for learning and memory. PACAP-related peptide interacts with three G-protein coupled receptors—VPAC1, VPAC2, and PACAP receptor (PAC1-R)—which mediate diverse biological effects. PAC1-R, a seven transmembrane protein, produces multiple isoforms through alternative splicing, each associated with distinct signaling pathways and expression patterns, further underscoring PACAP-related peptide complexity and importance in both normal physiology and pathological conditions.

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PACAP-related peptide Antibody (D-4) References:

1. Pituitary adenylate cyclase-activating polypeptide and its receptors: from structure to functions.  |  Vaudry, D., et al. 2000. Pharmacol Rev. 52: 269-324. PMID: 10835102
2. Overexpression of the human VPAC2 receptor in the suprachiasmatic nucleus alters the circadian phenotype of mice.  |  Shen, S., et al. 2000. Proc Natl Acad Sci U S A. 97: 11575-80. PMID: 11027354
3. Expression of vasoactive intestinal peptide (VIP) receptors in human uterus.  |  Bajo, AM., et al. 2000. Peptides. 21: 1383-8. PMID: 11072126
4. Vasoactive intestinal peptide: cardiovascular effects.  |  Henning, RJ. and Sawmiller, DR. 2001. Cardiovasc Res. 49: 27-37. PMID: 11121793
5. Selective gene expression and activation-dependent regulation of vasoactive intestinal peptide receptor type 1 and type 2 in human T cells.  |  Lara-Marquez, M., et al. 2001. J Immunol. 166: 2522-30. PMID: 11160313
6. Regulation of vasoactive intestinal peptide receptor expression in developing nervous systems.  |  Karacay, B., et al. 2000. Ann N Y Acad Sci. 921: 165-74. PMID: 11193820
7. Pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptors in the brain.  |  Shioda, S. 2000. Kaibogaku Zasshi. 75: 487-507. PMID: 11197592
8. Expression of pituitary adenylate cyclase-activating polypeptide 1 and 2 receptor mRNA in gallbladder tissue of patients with gallstone or gallbladder polyps.  |  Zhang, ZH., et al. 2006. World J Gastroenterol. 12: 1468-71. PMID: 16552823
9. The role of ADCYAP1, adenylate cyclase activating polypeptide 1, as a methylation biomarker for the early detection of cervical cancer.  |  Jung, S., et al. 2011. Oncol Rep. 25: 245-52. PMID: 21109983
10. Interaction between kisspeptin and adenylate cyclase-activating polypeptide 1 on the expression of pituitary gonadotropin subunits: a study using mouse pituitary lbetaT2 cells.  |  Mijiddorj, T., et al. 2017. Biol Reprod. 96: 1043-1051. PMID: 28863434
11. Adenylate cyclase-activating polypeptide 1 gene methylation predicts prognosis and the immune microenvironment of bladder cancer.  |  Xu, W., et al. 2021. Clin Transl Med. 11: e597. PMID: 34709747
12. Pituitary adenylate cyclase activating peptide-38 (PACAP-38), PACAP-27, and PACAP related peptide (PRP) in the rat median eminence and pituitary.  |  Mikkelsen, JD., et al. 1995. J Neuroendocrinol. 7: 47-55. PMID: 7735297

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PACAP-related peptide Antibody (D-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PACAP-related peptide Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PACAP-related peptide Antibody (D-4) Specifications

Alternate Product Name: Pituitary adenylate cyclase-activating polypeptide (PACAP)

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 75-103

Epitope Target: PACAP-related peptide

Epitope Species: human

Product Reactivity/Detection: precursor and processed active PACAP related peptide

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PACAP-related peptide Antibody (D-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PACAP antibody is PACAP Antibody (F-2): sc-166180.