PABP Antibody (10E10): sc-32318

PABP Antibody (10E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Poly(A)-binding protein (PABP) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-PABP antibody (10E10) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. PABP is a crucial, well-conserved multifunctional protein that plays a significant role in translational initiation, mRNA biogenesis, and degradation, making PABP essential for cellular function. Located predominantly in the cytoplasm, PABP interacts with polyadenylated mRNA, facilitating the recruitment of translation initiation factors and thereby promoting efficient mRNA translation. This localization ensures that protein synthesis machinery can access mRNA quickly, which is particularly important during cellular stress or in response to signaling pathways that regulate gene expression. The human PABP gene is mapped to chromosome 8q22.2-q23 and encodes a 633 amino acid protein, featuring four N-terminal RNA-binding domains and a large C-terminus involved in protein-protein interactions. Understanding PABP dynamics and interactions is critical, especially in viral infections, where poliovirus can cleave PABP, leading to host protein synthesis shutdown.

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PABP Antibody (10E10) References:

1. X-ray structure of the human hyperplastic discs protein: an ortholog of the C-terminal domain of poly(A)-binding protein.  |  Deo, RC., et al. 2001. Proc Natl Acad Sci U S A. 98: 4414-9. PMID: 11287654
2. Vasopressin mRNA localization in nerve cells: characterization of cis-acting elements and trans-acting factors.  |  Mohr, E., et al. 2001. Proc Natl Acad Sci U S A. 98: 7072-9. PMID: 11416190
3. Analysis of an essential requirement for the poly(A) binding protein function using cross-species complementation.  |  Chekanova, JA., et al. 2001. Curr Biol. 11: 1207-14. PMID: 11516954
4. Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation.  |  Svitkin, YV., et al. 2001. RNA. 7: 1743-52. PMID: 11780631
5. Efficient cleavage of ribosome-associated poly(A)-binding protein by enterovirus 3C protease.  |  Kuyumcu-Martinez, NM., et al. 2002. J Virol. 76: 2062-74. PMID: 11836384
6. Inhibition of translation and progesterone-induced maturation of Xenopus oocytes by expressing the amino-terminal portion of the eukaryotic translation initiation factor 4G.  |  Wakiyama, M. and Miura, K. 2002. Biosci Biotechnol Biochem. 66: 185-7. PMID: 11866104
7. LARP1 and LARP4: up close with PABP for mRNA 3' poly(A) protection and stabilization.  |  Mattijssen, S., et al. 2021. RNA Biol. 18: 259-274. PMID: 33522422
8. The flip-flop configuration of the PABP-dimer leads to switching of the translation function.  |  Gu, S., et al. 2022. Nucleic Acids Res. 50: 306-321. PMID: 34904669
9. PABP prevents the untimely decay of select mRNA populations in human cells.  |  Kajjo, S., et al. 2022. EMBO J. 41: e108650. PMID: 35156721
10. PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunity.  |  Wang, J., et al. 2022. Cell. 185: 3186-3200.e17. PMID: 35907403
11. A YTHDF-PABP interaction is required for m6 A-mediated organogenesis in plants.  |  Due Tankmar, M., et al. 2023. EMBO Rep. 24: e57741. PMID: 38009565
12. PABP-driven secondary condensed phase within RSV inclusion bodies activates viral mRNAs for ribosomal recruitment.  |  Zhang, Q., et al. 2024. Virol Sin. 39: 235-250. PMID: 38072230

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PABP Antibody (10E10) has 93 citations in a variety of scientific publications.

Primary antibodies like Anti-PABP Antibody (10E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PABP Antibody (10E10) Specifications

Alternate Product Name: PABP

Product Clone Name: 10E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PABP

Epitope Species: human

Product Reactivity/Detection: PABP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: bovine and canine

Additional Non-Mammalian Reactivity: Xenopus

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 93 citations in various scientific publications

Available Conjugates/Forms for PABP Antibody (10E10): AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790