PA28α Antibody (AT12H3): sc-517419

PA28α Antibody (AT12H3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PA28α in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PA28α antibody (AT12H3) is available as a non-conjugated format. PA28α plays a crucial role in immune response as an interferon γ (IFNγ) inducible proteasome activator essential for presenting certain major histocompatibility class I antigens. PA28α is located in the cytoplasm and nucleus, where PA28α associates with the 20S proteasome to enhance its activity, facilitating the degradation of ubiquitinated proteins and generating peptide fragments for MHC class I presentation. The PA28 complex, composed of PA28α and PA28β, forms a hexameric ring structure that binds to both ends of the proteasome, significantly increasing efficiency in processing proteins. PA28α is vital for maintaining immune surveillance and response, as downregulation can lead to abnormal proteasome activation, implicated in various pathological conditions, including intimal hyperplasia. The PMSE2 gene, which encodes the β-subunit of proteasome activator PA28, is located on chromosome 14q11.2, highlighting the genetic basis for expression and function in immune regulation.

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PA28α Antibody (AT12H3) References:

1. Interferon gamma regulates accumulation of the proteasome activator PA28 and immunoproteasomes at nuclear PML bodies.  |  Fabunmi, RP., et al. 2001. J Cell Sci. 114: 29-36. PMID: 11112687
2. Properties of the beta subunit of the proteasome activator PA28 (11S REG).  |  Wilk, S., et al. 2000. Arch Biochem Biophys. 384: 174-80. PMID: 11147828
3. Immunoproteasome assembly and antigen presentation in mice lacking both PA28alpha and PA28beta.  |  Murata, S., et al. 2001. EMBO J. 20: 5898-907. PMID: 11689430
4. Relationship of the 20S proteasome and the proteasome activator PA28 to atherosclerosis and intimal hyperplasia in the human vascular system.  |  Faries, PL., et al. 2001. Ann Vasc Surg. 15: 628-33. PMID: 11769143
5. Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes.  |  Kohda, K., et al. 1998. J Immunol. 160: 4923-35. PMID: 9590240

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PA28α Antibody (AT12H3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PA28α Antibody (AT12H3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PA28α Antibody (AT12H3) Specifications

Alternate Product Name: PA28α

Product Clone Name: AT12H3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-249

Epitope Target: PA28α

Epitope Species: human

Product Reactivity/Detection: PA28α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 1% glycerol, and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PA28α Antibody (AT12H3): N/A

The preferred PA28α antibody is PA28α Antibody (G-9): sc-518190.