p54/nrb Antibody (F-5) recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p54/nrb Antibody (F-5): sc-376804

p54/nrb Antibody (F-5) is rated 4.3 out of 5 by 6.
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Datasheets

    • Anti-p54/nrb Antibody (F-5) is a mouse monoclonal IgG1 p54/nrb antibody provided at 200 µg/ml
    • raised against amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin
    • Anti-p54/nrb Antibody (F-5) is recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-p54/nrb Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-376804 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p54/nrb (F-5): sc-376804.
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Can p54/nrb (F-5): sc-376804 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, p54/nrb (F-5): sc-376804 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from No specific staining in immunofluorescence I tested three antibodies for IF. F-5 does not work in my conditions.
Date published: 2019-10-18
Rated 5 out of 5 by from Excellent product Got very good results for immunofluorescence. Very helpful!
Date published: 2017-09-17
Rated 5 out of 5 by from Great features! I bought this for immunohistochemistry,and got a good results!
Date published: 2017-06-20
Rated 5 out of 5 by from excellent antibody for WB in rat tissue NE Strong detection of p54/nrb in WB of rat Liver, Spleen, Lung, Kidney, Testis.
Date published: 2017-03-19
Rated 5 out of 5 by from Superb immunofluorescence nuclear staining Superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-19
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