p54/nrb Antibody (E-3): sc-376806

p54/nrb Antibody (E-3) is a mouse monoclonal IgG2b antibody that detects p54/nrb in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p54/nrb, a nuclear RNA-binding protein, plays a crucial role in the formation and function of paraspeckles, which are essential for the regulation of gene expression and RNA processing. These structures are located in the interchromatin nucleoplasmic space and are involved in the storage and processing of non-coding RNAs, thereby influencing the cellular response to stress and transcriptional regulation. The dynamic movement of p54/nrb between the nucleolus and paraspeckles highlights p54/nrb′s importance in cellular signaling pathways, particularly during transcriptional inhibition when p54/nrb translocates to distinct caps at the nucleolar periphery. p54/nrb contains two RNA recognition motifs and shares significant homology with the human splicing factor PSF and Drosophila NONA/BJ6, which is vital for normal vision and courtship behaviors. The shared DBHS (Drosophila behavior, human splicing) domain among these proteins suggests a conserved mechanism in regulating pre-mRNA splicing pathways, making p54/nrb a key player in maintaining cellular homeostasis and proper gene expression.

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p54/nrb Antibody (E-3) References:

1. p54nrb/NONO regulates cyclic AMP-dependent glucocorticoid production by modulating phosphodiesterase mRNA splicing and degradation.  |  Lu, JY. and Sewer, MB. 2015. Mol Cell Biol. 35: 1223-37. PMID: 25605330
2. Tyrosine Residues Regulate Multiple Nuclear Functions of P54nrb.  |  Lee, AR., et al. 2017. J Cell Physiol. 232: 852-861. PMID: 27430900
3. NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation.  |  Lahaye, X., et al. 2018. Cell. 175: 488-501.e22. PMID: 30270045
4. An RNA-binding-protein, NONO governs energy metabolism by regulating NAMPT in lung cancer.  |  Kim, SJ., et al. 2020. Biochem Biophys Res Commun. 528: 376-382. PMID: 32087970
5. NONO Inhibits Lymphatic Metastasis of Bladder Cancer via Alternative Splicing of SETMAR.  |  Xie, R., et al. 2021. Mol Ther. 29: 291-307. PMID: 32950106
6. Long Noncoding RNA FAM225A Promotes Esophageal Squamous Cell Carcinoma Development and Progression via Sponging MicroRNA-197-5p and Upregulating NONO.  |  Zhu, P., et al. 2021. J Cancer. 12: 1073-1084. PMID: 33442405
7. NONO regulates B-cell development and B-cell receptor signaling.  |  Zhang, Y., et al. 2023. FASEB J. 37: e22862. PMID: 36906291
8. LncRNA like NMRK2 mRNA functions as a key molecular scaffold to enhance mitochondrial respiration of NONO-TFE3 rearranged renal cell carcinoma in an NAD+ kinase-independent manner.  |  Chen, Y., et al. 2023. J Exp Clin Cancer Res. 42: 252. PMID: 37770905
9. NONO promotes gallbladder cancer cell proliferation by enhancing oncogenic RNA splicing of DLG1 through interaction with IGF2BP3/RBM14.  |  Yang, ZY., et al. 2024. Cancer Lett. 587: 216703. PMID: 38341127
10. AFX1 and p54nrb: fine mapping, genomic structure, and exclusion as candidate genes of X-linked dystonia parkinsonism.  |  Peters, U., et al. 1997. Hum Genet. 100: 569-72. PMID: 9341872

The preferred p54/nrb antibody is p54/nrb Antibody (G-1): sc-376865.