p54/nrb Antibody (B-3): sc-377421

p54/nrb Antibody (B-3) is a mouse monoclonal IgG1 antibody that detects p54/nrb in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p54/nrb, a nuclear RNA-binding protein, plays a crucial role in gene expression regulation by participating in paraspeckle formation, which are subnuclear structures involved in RNA storage and processing. These paraspeckles are essential for proper RNA localization and transcriptional activity regulation, particularly under stress conditions. The dynamic movement of p54/nrb between the nucleolus and paraspeckles highlights its importance in cellular responses to transcriptional inhibition, as p54/nrb translocates to distinct caps in the nucleolar periphery. p54/nrb contains two RNA recognition motifs and shares significant homology with human splicing factor PSF and Drosophila NONA/BJ6, which is vital for normal vision and courtship behaviors. The shared DBHS (Drosophila behavior, human splicing) domain among these proteins suggests a potential role in regulating pre-mRNA splicing pathways, making p54/nrb a key player in RNA processing and gene regulation. With broad species reactivity and versatility in various applications, anti-p54/nrb antibody (B-3) serves as an invaluable tool for researchers studying RNA biology and gene expression mechanisms.

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p54/nrb Antibody (B-3) References:

1. Nuclear NonO/p54(nrb) protein is a nonclassical carbonic anhydrase.  |  Karhumaa, P., et al. 2000. J Biol Chem. 275: 16044-9. PMID: 10821857
2. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner.  |  Fox, AH., et al. 2005. Mol Biol Cell. 16: 5304-15. PMID: 16148043
3. TNF-alpha suppresses prolyl-4-hydroxylase alpha1 expression via the ASK1-JNK-NonO pathway.  |  Zhang, C., et al. 2007. Arterioscler Thromb Vasc Biol. 27: 1760-7. PMID: 17478756
4. A coactivator trap identifies NONO (p54nrb) as a component of the cAMP-signaling pathway.  |  Amelio, AL., et al. 2007. Proc Natl Acad Sci U S A. 104: 20314-9. PMID: 18077367
5. Transcriptional activity of the murine retinol-binding protein gene is regulated by a multiprotein complex containing HMGA1, p54 nrb/NonO, protein-associated splicing factor (PSF) and steroidogenic factor 1 (SF1)/liver receptor homologue 1 (LRH-1).  |  Bianconcini, A., et al. 2009. Int J Biochem Cell Biol. 41: 2189-203. PMID: 19389484
6. Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation.  |  Ainaoui, N., et al. 2015. PLoS One. 10: e0136466. PMID: 26332123
7. Melanotic PEComa of the Sinonasal Mucosa With NONO-TFE3 Fusion: An Elusive Mimic of Sinonasal Melanoma.  |  McGregor, SM., et al. 2017. Am J Surg Pathol. 41: 717-722. PMID: 28009605
8. The RNA-Binding Protein NONO Coordinates Hepatic Adaptation to Feeding.  |  Benegiamo, G., et al. 2018. Cell Metab. 27: 404-418.e7. PMID: 29358041
9. NONO enhances mRNA processing of super-enhancer-associated GATA2 and HAND2 genes in neuroblastoma.  |  Zhang, S., et al. 2023. EMBO Rep. 24: e54977. PMID: 36416237
10. Nono deficiency impedes the proliferation and adhesion of H9c2 cardiomyocytes through Pi3k/Akt signaling pathway.  |  Lei, YQ., et al. 2023. Sci Rep. 13: 7134. PMID: 37130848

The preferred p54/nrb antibody is p54/nrb Antibody (G-1): sc-376865.