p47-phox Antibody (D-10) recommended for detection of p47-phox of mouse, rat and human origin by WB, IP, IF and ELISA

p47-phox Antibody (D-10): sc-17845

p47-phox Antibody (D-10) is rated 4.7 out of 5 by 11.
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Datasheets

    • Anti-p47-phox Antibody (D-10) is a mouse monoclonal IgG1 p47-phox antibody provided at 200 µg/ml
    • raised against amino acids 196-390 of p47-phox of human origin
    • Anti-p47-phox Antibody (D-10) is recommended for detection of p47-phox of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-p47-phox Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p47-phox (D-10): sc-17845.
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Can it detect the phosphorylation of p47phox in RAW264.7 after PMA stimulation by SDS-pages? How can I get a free 10ug samples? Thank you

Asked by: Mark1
Thank you for your question. This mouse monoclonal antibody, p47-phox (D-10): sc-17845, is not phospho-specific but it should be able to detect both the phosphorylated and non-phosphorylated forms of p47-phox in mouse samples. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-11-14

Can you recommend a positive control to use with p47-phox Antibody (D-10): sc-17845 for Western blot?

Asked by: jenni
We recommend Ramos: sc-2216, Daudi: sc-2415, HL-60: sc-2209, THP-1 : sc-2238 or RAW 264.7: sc-2211 whole cell lysates to use as positive controls as we have Western blot data showing specific detection of p47-phox in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-09
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Rated 5 out of 5 by from specific band Loading 40 ug mouse spleen tissue lysates each lane.The specific band is at 47kda.Recommend use dilution 1:500 to 1:800.
Date published: 2017-08-26
Rated 5 out of 5 by from IB signal is strong and back ground is clear. I have tried this antibody with neutrophil lysate. The band is clear and no back groud. Very recommend.
Date published: 2017-08-15
Rated 4 out of 5 by from Quite good antibody We tested p47phox by western blotting analysis. 60 micrograms of mouse brain membrane lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal p47phox antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). p47phox signal was detected by chemidoc (Biorad) and the signal was reached only after long exposition. Based on our experience p47phox is a quite good antibody.
Date published: 2017-07-20
Rated 5 out of 5 by from antibody results This mono clone gives specific and clean WB, Great antibody!
Date published: 2017-06-01
Rated 5 out of 5 by from good quality It was used to detect p47-phox in rat brain. Bands were clear.
Date published: 2017-04-19
Rated 5 out of 5 by from Great antibody! The antibody for the WB of rat protein,the band is clear and special!
Date published: 2017-03-15
Rated 5 out of 5 by from Very Efficient p47-phox antibody very useful in the experiment. The analysis results of Western blot were very strong and clear.
Date published: 2017-02-06
Rated 5 out of 5 by from Outstanding western data Very nice western blot results; strong signal, low background
Date published: 2017-01-07
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