p47phox Antibody (A-7): sc-17844
- p47phox Antibody (A-7) is a mouse monoclonal IgG1 κ, cited in 43 publications, provided at 200 µg/ml
- raised against amino acids 196-390 of p47phox of human origin
- Anti-p47phox Antibody (A-7) is recommended for detection of p47phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-p47phox Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for p47phox Antibody (A-7) for WB and IHC(P) applications. This reagent is now offered in a bundle with p47phox Antibody (A-7) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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p47phox Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects p47phox of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-p47phox antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p47phox plays a crucial role in the immune response by participating in the activation of the NADPH oxidase complex, which is essential for the production of reactive oxygen species in neutrophils and other phagocytic cells. This process is vital for the destruction of pathogens, and mutations in p47phox have been linked to chronic granulomatous disease, a hereditary condition that impairs the immune system′s ability to fight infections. p47phox structure includes two Src homology 3 (SH3) domains, which are important for interaction with p67-phox, another key component of the NADPH oxidase complex. The C-terminal SH3 domain of p67-phox interacts with the proline-rich domain of p47phox, facilitating the transport of p67-phox to the plasma membrane during enzyme activation. This interaction underscores p47phox importance in the assembly and function of the NADPH oxidase complex, making p47phox a critical target for research in immunology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p47phox Antibody (A-7) References:
- p47phox, the phagocyte NADPH oxidase/NOX2 organizer: structure, phosphorylation and implication in diseases. | El-Benna, J., et al. 2009. Exp Mol Med. 41: 217-25. PMID: 19372727
- Mechanosensitive regulation of cortactin by p47phox: a new paradigm in cytoskeletal remodeling. | Montezano, AC. and Touyz, RM. 2013. Circ Res. 112: 1522-5. PMID: 23743221
- p47phox siRNA-Loaded PLGA Nanoparticles Suppress ROS/Oxidative Stress-Induced Chondrocyte Damage in Osteoarthritis. | Shin, HJ., et al. 2020. Polymers (Basel). 12: PMID: 32069893
- p47phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis. | Wang, X., et al. 2020. Redox Biol. 34: 101569. PMID: 32422541
- Impaired p47phox phosphorylation in neutrophils from patients with p67phox-deficient chronic granulomatous disease. | Belambri, SA., et al. 2022. Blood. 139: 2512-2522. PMID: 35108370
- A dual Keap1 and p47phox inhibitor Ginsenoside Rb1 ameliorates high glucose/ox-LDL-induced endothelial cell injury and atherosclerosis. | Wang, ZC., et al. 2022. Cell Death Dis. 13: 824. PMID: 36163178
- ROCK2 interacts with p22phox to phosphorylate p47phox and to control NADPH oxidase activation in human monocytes. | Tlili, A., et al. 2023. Proc Natl Acad Sci U S A. 120: e2209184120. PMID: 36626553
- Targeting NOX2 with Bivalent Small-Molecule p47phox-p22phox Inhibitors. | Zang, J., et al. 2023. J Med Chem. 66: 14963-15005. PMID: 37857466
- Allogeneic hematopoietic cell transplantation is effective for p47phox chronic granulomatous disease: A Primary Immune Deficiency Treatment Consortium study. | Grunebaum, E., et al. 2024. J Allergy Clin Immunol. 153: 1423-1431.e2. PMID: 38290608
- Circ_005077 accelerates myocardial lipotoxicity induced by high-fat diet via CyPA/p47PHOX mediated ferroptosis. | Ni, X., et al. 2024. Cardiovasc Diabetol. 23: 129. PMID: 38622592
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p47phox Antibody (A-7) | sc-17844 | 200 µg/ml | $322.00 | |||
p47phox Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-520682 | 200 µg Ab, 40 µg BP | $361.00 | |||
p47phox Antibody (A-7) AC | sc-17844 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p47phox Antibody (A-7) HRP | sc-17844 HRP | 200 µg/ml | $322.00 | |||
p47phox Antibody (A-7) FITC | sc-17844 FITC | 200 µg/ml | $336.00 | |||
p47phox Antibody (A-7) PE | sc-17844 PE | 200 µg/ml | $349.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 488 | sc-17844 AF488 | 200 µg/ml | $364.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 546 | sc-17844 AF546 | 200 µg/ml | $364.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 594 | sc-17844 AF594 | 200 µg/ml | $364.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 647 | sc-17844 AF647 | 200 µg/ml | $364.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 680 | sc-17844 AF680 | 200 µg/ml | $364.00 | |||
p47phox Antibody (A-7) Alexa Fluor® 790 | sc-17844 AF790 | 200 µg/ml | $364.00 |