p38 alpha Antibody (A1F7) recommended for detection of p38α of human origin by WB, IP, IF and IHC(P)

p38α Antibody (A1F7): sc-33688

p38α Antibody (A1F7) is rated 4.4 out of 5 by 5.
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Datasheets

    • p38α Antibody (A1F7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant p38 of human origin
    • recommended for detection of p38α of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p38α/β (A-12): sc-7972 for p38α antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can p38α (A1F7): sc-33688 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, p38α (A1F7): sc-33688 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great features This antibody worked great for western blot and images are very good.
Date published: 2019-03-29
Rated 5 out of 5 by from Publications describe success by Western Publications describe success by Western Blot using this antibody to analyze Jurkat and SK-N-SH cells. -SCBT Publication Review
Date published: 2015-05-01
Rated 5 out of 5 by from Publishable data obtained by Western Blot Publishable data obtained by Western Blot using human airway smooth muscle tissue. -SCBT Publication Review
Date published: 2015-04-04
Rated 4 out of 5 by from A definite increase in signal was observed A definite increase in signal was observed by Western Blot when comparing human p38 transfected 293T cells with those that have not been transfected to over express this particular protein. -SCBT QC
Date published: 2015-01-09
Rated 3 out of 5 by from Strong band observed via Western Blot using Strong band observed via Western Blot using human recombinant p38 . -SCBT QC
Date published: 2014-01-21
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