Waf1/Cip1/CDKN1A p21 Antibody (WA-1): sc-51689

p21 Waf1/Cip1 Antibody (WA-1) is a mouse monoclonal IgG1 antibody that recognizes p21 Waf1/Cip1 of human origin, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p21 Waf1/Cip1 plays a crucial role in cell cycle regulation by acting as a potent inhibitor of cyclin-dependent kinases (Cdks), which are essential for the progression of the cell cycle. By binding tightly to Cdks, p21 Waf1/Cip1 prevents the phosphorylation of key substrates like retinoblastoma protein (Rb), thereby halting the transition from the G1 phase to the S phase of the cell cycle. This regulatory function is particularly important in cellular responses to stress and DNA damage, where p21 Waf1/Cip1 mediates the p53-dependent checkpoint activation, ensuring damaged DNA is repaired before replication or triggering senescence or apoptosis if the damage is irreparable. The induction of p21 Waf1/Cip1 following stress signals not only halts proliferation but also interacts with proliferating cell nuclear antigen (PCNA) to influence DNA repair mechanisms. By tightly integrating DNA damage response pathways with cell cycle control, p21 Waf1/Cip1 acts as a guardian of genomic integrity, highlighting its importance in preventing tumorigenesis. Anti-p21 Waf1/Cip1 antibody (WA-1) is an essential tool for studying these intricate mechanisms and the role of p21 Waf1/Cip1 in various physiological and pathological processes.

The preferred Waf1/Cip1/CDKN1A p21 antibody is Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246.