Waf1/Cip1/CDKN1A p21 Antibody (DCS-60): sc-56335

p21 Waf1/Cip1 Antibody (DCS-60) is a mouse monoclonal IgG2a antibody designed to detect the full length p21 Waf1/Cip1 of human origin, also known as CDK-interacting protein 1 (CDKN1A) or WAF1, in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p21 Waf1/Cip1 is a critical regulator of the cell cycle, functioning as an inhibitor of cyclin-dependent kinases (Cdks) to block the phosphorylation of retinoblastoma protein (Rb) and halt cell cycle progression from the G1 to the S phase. This function is vital for maintaining cellular integrity by allowing time for DNA repair or initiating cell cycle arrest in response to stress signals. p21 Waf1/Cip1 is predominantly localized in the nucleus, where p21 Waf1/Cip1 interacts with key regulatory proteins to influence cell cycle dynamics, DNA replication, and repair processes. Nuclear localization is crucial for suppressing tumorigenesis, as dysregulation of p21 Waf1/Cip1 expression or function can result in unchecked cell proliferation. Expression of p21 Waf1/Cip1 is primarily controlled by the tumor suppressor p53, which induces upregulation following DNA damage, linking p21 Waf1/Cip1 to stress response pathways essential for genomic stability. Conservation of p21 Waf1/Cip1 across species, with the mouse homolog known as CAP20, emphasizes a fundamental role in cell cycle control and stress response mechanisms. p21 Waf1/Cip1 (DCS-60) antibody is an indispensable resource for studying these intricate regulatory pathways and their implications in cancer biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Waf1/Cip1/CDKN1A p21 Antibody (DCS-60) References:

1. Senescence-like changes induced by expression of p21(waf1/Cip1) in NIH3T3 cell line.  |  Chen, X., et al. 2002. Cell Res. 12: 229-33. PMID: 12296382
2. Mist1 regulates pancreatic acinar cell proliferation through p21 CIP1/WAF1.  |  Jia, D., et al. 2008. Gastroenterology. 135: 1687-97. PMID: 18762186
3. Co-expression of p21(Waf1/Cip1) in adenovirus vectors improves expression of a second transgene.  |  Schumacher, A., et al. 2009. Gene Ther. 16: 574-8. PMID: 19225550
4. Induction of p21(CIP1/WAF1) expression by human T-lymphotropic virus type 1 Tax requires transcriptional activation and mRNA stabilization.  |  Zhang, L., et al. 2009. Retrovirology. 6: 35. PMID: 19356250
5. Chondrocyte p21(WAF1/CIP1) expression is increased by dexamethasone but does not contribute to dexamethasone-induced growth retardation in vivo.  |  Owen, HC., et al. 2009. Calcif Tissue Int. 85: 326-34. PMID: 19727539
6. Estrogen regulated expression of the p21 Waf1/Cip1 gene in estrogen receptor positive human breast cancer cells.  |  Mandal, S. and Davie, JR. 2010. J Cell Physiol. 224: 28-32. PMID: 20301197
7. p21Cip1/Waf1 protein and its function based on a subcellular localization [corrected].  |  Cmielová, J. and Rezáčová, M. 2011. J Cell Biochem. 112: 3502-6. PMID: 21815189
8. Combined loss of p21(waf1/cip1) and p27(kip1) enhances tumorigenesis in mice.  |  García-Fernández, RA., et al. 2011. Lab Invest. 91: 1634-42. PMID: 21876534
9. ATR controls the p21(WAF1/Cip1) protein up-regulation and apoptosis in response to low UV fluences.  |  Al-Khalaf, HH., et al. 2012. Mol Carcinog. 51: 930-8. PMID: 21976111

The preferred Waf1/Cip1/CDKN1A p21 antibody is Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246.