Waf1/Cip1/CDKN1A p21 Antibody (B-2): sc-271532

p21 Waf1/Cip1 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects an epitope mapping between amino acids 124-164 at the C-terminus of p21 Waf1/Cip1 of human origin, also known as CDKN1A, Cip1, or WAF1, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p21 is a critical regulator of the cell cycle, acting as an inhibitor of cyclin-dependent kinases (Cdks) to prevent the phosphorylation of retinoblastoma protein (Rb) by cyclin-Cdk complexes. This function halts cell cycle progression, particularly from the G1 phase to the S phase, allowing the cell to repair DNA damage or respond to stress signals by promoting senescence or apoptosis. p21 Waf1/Cip1 (B-2) antibody expression is tightly regulated by the tumor suppressor protein p53, which induces p21 in response to DNA damage, linking p21 to critical cellular mechanisms that maintain genomic stability and prevent tumorigenesis. p21 Waf1/Cip1 monoclonal antibody (B-2) also interacts with proliferating cell nuclear antigen (PCNA), influencing DNA replication and repair processes, further emphasizing p21′s importance in cellular homeostasis. The evolutionary conservation of p21, reflected in the mouse homolog known as CAP20, underscores p21′s fundamental role across species in regulating cell proliferation. p21′s central role in coordinating cell cycle arrest and stress responses makes anti-p21 antibody (B-2) a valuable tool for studying these processes in detail.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Waf1/Cip1/CDKN1A p21 Antibody (B-2) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-Waf1/Cip1/CDKN1A p21 Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Waf1/Cip1/CDKN1A p21 Antibody (B-2) Specifications

Alternate Product Name: p21 Waf1/Cip1

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 124-164

Epitope Target: p21 Waf1/Cip1

Epitope Species: human

Product Reactivity/Detection: p21 Waf1/Cip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for Waf1/Cip1/CDKN1A p21 Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Waf1/Cip1/CDKN1A p21 antibody is Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246.