Waf1/Cip1/CDKN1A p21 Antibody (0.N.488): sc-71811

p21 Waf1/Cip1 Antibody (0.N.488) is a mouse monoclonal IgG2a antibody raised against full length p21 Waf1/Cip1 of human origin, also known as CDKN1A, Cip1, or WAF1, in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p21 is a critical cell cycle regulator that inhibits cyclin-dependent kinases (Cdks), thereby preventing the phosphorylation of retinoblastoma protein (Rb) and halting the progression of the cell cycle from the G1 phase to the S phase. This inhibition allows cells to respond to stress and DNA damage, with p21 expression being directly regulated by the tumor suppressor p53. p21 interacts with several key proteins, including proliferating cell nuclear antigen (PCNA), which links p21 to DNA replication and repair processes, and various cyclin-Cdk complexes such as cyclin A-Cdk2 and cyclin E-Cdk2, underscoring p21′s role in coordinating cellular responses to genomic instability. Furthermore, p21′s ability to form complexes with transcription factors like E2F1 highlights p21′s broader impact on cell cycle control and gene regulation. Dysregulation of these interactions can contribute to uncontrolled cell proliferation, making p21 a vital focus in cancer biology. Anti-p21 Waf1/Cip1 antibody (0.N.488) offers a robust tool for researchers studying these intricate interactions and their implications for tumor suppression and cell cycle regulation.

The preferred Waf1/Cip1/CDKN1A p21 antibody is Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246.