p-SMC1 Antibody (5D11G5): sc-56746

p-SMC1 Antibody (5D11G5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 957 phosphorylated SMC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-p-SMC1 antibody (5D11G5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SMC1, part of the structural maintenance of chromosomes family, plays a crucial role in maintaining sister chromatid cohesion and facilitating chromosome condensation during mitosis, which is essential for accurate cell division. The SMC1/SMC3 complex is particularly important for metaphase progression in mitotic cells, functioning independently of the SMC2/SMC4 complex throughout the cell cycle. SMC1 is ubiquitously expressed across various human tissues, including the thymus, testis, and colon, highlighting a fundamental role in cellular processes. Understanding SMC1 phosphorylation dynamics is vital for elucidating mechanisms of cell cycle regulation and potential implications in cancer biology, where dysregulation of these processes can lead to tumorigenesis.

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p-SMC1 Antibody (5D11G5) References:

1. Isolation and characterization of a human cDNA homologous to the Xenopus laevis XCAP-C gene belonging to the structural maintenance of chromosomes (SMC) family.  |  Nishiwaki, T., et al. 1999. J Hum Genet. 44: 197-202. PMID: 10319587
2. Complete cDNA cloning, genomic organization, chromosomal assignment, functional characterization of the promoter, and expression of the murine Bamacan gene.  |  Ghiselli, G., et al. 1999. J Biol Chem. 274: 17384-93. PMID: 10358101
3. Structural maintenance of chromosomes (SMC) proteins: conserved molecular properties for multiple biological functions.  |  Strunnikov, AV. and Jessberger, R. 1999. Eur J Biochem. 263: 6-13. PMID: 10429180
4. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint.  |  Yazdi, PT., et al. 2002. Genes Dev. 16: 571-82. PMID: 11877377
5. Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA double-strand breaks in human cells.  |  Bauerschmidt, C., et al. 2011. Exp Cell Res. 317: 330-7. PMID: 21056556
6. Phosphorylation of SMC1 by ATR is required for desferrioxamine (DFO)-induced apoptosis.  |  So, EY., et al. 2011. Cell Death Dis. 2: e128. PMID: 21390062
7. Radiation-Induced Phosphorylation of Serine 360 of SMC1 in Human Peripheral Blood Mononuclear Cells.  |  Park, S., et al. 2019. Radiat Res. 191: 262-270. PMID: 30702968
8. The human SB1.8 gene (DXS423E) encodes a putative chromosome segregation protein conserved in lower eukaryotes and prokaryotes.  |  Rocques, PJ., et al. 1995. Hum Mol Genet. 4: 243-9. PMID: 7757074
9. SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.  |  Strunnikov, AV., et al. 1993. J Cell Biol. 123: 1635-48. PMID: 8276886
10. Basement membrane abnormalities in human eyes with diabetic retinopathy.  |  Ljubimov, AV., et al. 1996. J Histochem Cytochem. 44: 1469-79. PMID: 8985139
11. cDNA cloning of the basement membrane chondroitin sulfate proteoglycan core protein, bamacan: a five domain structure including coiled-coil motifs.  |  Wu, RR. and Couchman, JR. 1997. J Cell Biol. 136: 433-44. PMID: 9015313
12. Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.  |  Schmiesing, JA., et al. 1998. Proc Natl Acad Sci U S A. 95: 12906-11. PMID: 9789013

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-SMC1 Antibody (5D11G5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-p-SMC1 Antibody (5D11G5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-SMC1 Antibody (5D11G5) Specifications

Alternate Product Name: p-SMC1

Product Clone Name: 5D11G5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 957

Epitope Target: SMC1

Epitope Species: human

Product Reactivity/Detection: Ser 957 phosphorylated SMC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for p-SMC1 Antibody (5D11G5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SMC1 antibody is SMC1 Antibody (C2M): sc-56776.