P-Selectin Antibody (AK4) recommended for detection of P-Selectin of human origin by WB, IP, IF and FCM

P-Selectin Antibody (AK4): sc-19996

P-Selectin Antibody (AK4) is rated 4.3 out of 5 by 3.
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Datasheets

    • P-Selectin Antibody (AK4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against carbohydrate recognition domain (CRD) of P-Selectin of human origin
    • recommended for detection of P-Selectin of human origin by WB, IP, IF and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also provided azide-free for blocking, sc-19996 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See P-Selectin (CTB201): sc-8419 for P-Selectin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can P-Selectin (AK4): sc-19996 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, P-Selectin (AK4): sc-19996 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for flow cytometry Great antibody for flow cytometry, good data with platelets. -SCBT Publication Review
Date published: 2015-05-08
Rated 4 out of 5 by from Large WB band produced with human platelet extract Large WB band produced with human platelet extract. -SCBT QC
Date published: 2015-04-19
Rated 4 out of 5 by from Robust FCM data of human platelets Robust FCM data of human platelets. -SCBT QC
Date published: 2013-03-23
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