p-Rb Antibody (F-4): sc-377540
- p-Rb Antibody (F-4) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- p-Rb Antibody (F-4) is recommended for detection of Ser 608 phosphorylated Rb of human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and avian
- Anti-p-Rb Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for p-Rb Antibody (F-4) for WB applications. This reagent is now offered in a bundle with p-Rb Antibody (F-4) (see ordering information below).
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SEE ALSO...
p-Rb Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ser 608 phosphorylated Rb of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Rb (F-4) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinoblastoma protein (Rb) plays a crucial role in regulating cell cycle, particularly at G1-S transition, by acting as a transcriptional repressor. Rb′s ability to control cell proliferation prevents uncontrolled cell growth, which can lead to cancer. Rb achieves this by binding to E2F transcription factors, inhibiting their activity, and preventing expression of genes necessary for S phase entry. Phosphorylation of Rb at specific serine residues, such as Ser 795, during G1 phase leads to inactivation, allowing cell cycle progression. This phosphorylation serves as a key regulatory mechanism and highlights the importance of post-translational modifications in Rb′s functional dynamics, as modifications dictate interactions with other proteins and role in tumor suppression. Understanding Rb regulation through phosphorylation remains essential for developing targeted therapies in cancers, such as retinoblastoma, where Rb gene mutations disrupt normal function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Rb Antibody (F-4) | sc-377540 | 200 µg/ml | $322.00 | |||
p-Rb Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-526111 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Rb Antibody (F-4) AC | sc-377540 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-Rb Antibody (F-4) HRP | sc-377540 HRP | 200 µg/ml | $322.00 | |||
p-Rb Antibody (F-4) FITC | sc-377540 FITC | 200 µg/ml | $336.00 | |||
p-Rb Antibody (F-4) PE | sc-377540 PE | 200 µg/ml | $349.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 488 | sc-377540 AF488 | 200 µg/ml | $364.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 546 | sc-377540 AF546 | 200 µg/ml | $364.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 594 | sc-377540 AF594 | 200 µg/ml | $364.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 647 | sc-377540 AF647 | 200 µg/ml | $364.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 680 | sc-377540 AF680 | 200 µg/ml | $364.00 | |||
p-Rb Antibody (F-4) Alexa Fluor® 790 | sc-377540 AF790 | 200 µg/ml | $364.00 | |||
p-Rb (F-4) Neutralizing Peptide | sc-377540 P | 100 µg/0.5 ml | $69.00 |