p-Rb Antibody (E-10): sc-271930
- p-Rb Antibody (E-10) is a mouse monoclonal IgG3 κ, cited in 17 publications, provided at 200 µg/ml
- recommended for detection of Thr 821 and Thr 826 dually phosphorylated Rb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-p-Rb Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for p-Rb Antibody (E-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-Rb Antibody (E-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SEE ALSO...
p-Rb Antibody (E-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects p-Rb, specifically the dually phosphorylated forms at Thr 821 and Thr 826, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Rb (E-10) antibody is offered in both non-conjugated and various conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinoblastoma protein (Rb) plays a crucial role in regulating the cell cycle, particularly at the G1-S transition, by acting as a tumor suppressor. Rb function is vital for maintaining cellular homeostasis, preventing excessive cell proliferation and promoting differentiation and apoptosis. Phosphorylation of Rb, particularly at Ser 795, leads to inactivation, allowing cell cycle progression. This phosphorylation is part of a complex regulatory mechanism involving multiple phosphorylation sites, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811, and Thr 821/Thr 826, which collectively modulate Rb activity. Hypophosphorylated Rb represses transcription of cell cycle-related genes through direct protein-protein interactions and recruitment of histone deacetylases, underscoring Rb importance in tumor suppression and oncogenesis prevention, making anti-p-Rb antibody (E-10) an essential tool for researchers studying cancer biology and cell cycle regulation.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Rb Antibody (E-10) | sc-271930 | 200 µg/ml | $316.00 | |||
p-Rb Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-522104 | 200 µg Ab, 40 µg BP | $354.00 | |||
p-Rb Antibody (E-10) AC | sc-271930 AC | 500 µg/ml, 25% agarose | $416.00 | |||
p-Rb Antibody (E-10) HRP | sc-271930 HRP | 200 µg/ml | $316.00 | |||
p-Rb Antibody (E-10) FITC | sc-271930 FITC | 200 µg/ml | $330.00 | |||
p-Rb Antibody (E-10) PE | sc-271930 PE | 200 µg/ml | $343.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 488 | sc-271930 AF488 | 200 µg/ml | $357.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 546 | sc-271930 AF546 | 200 µg/ml | $357.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 594 | sc-271930 AF594 | 200 µg/ml | $357.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 647 | sc-271930 AF647 | 200 µg/ml | $357.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 680 | sc-271930 AF680 | 200 µg/ml | $357.00 | |||
p-Rb Antibody (E-10) Alexa Fluor® 790 | sc-271930 AF790 | 200 µg/ml | $357.00 | |||
p-Rb (E-10) Neutralizing Peptide | sc-271930 P | 100 µg/0.5 ml | $68.00 |