p-Rb Antibody (B-4): sc-514031

p-Rb Antibody (B-4) is a mouse monoclonal IgM kappa antibody that detects Ser 795 phosphorylated Rb of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Rb (B-4) antibody is available in a non-conjugated form, specifically designed for detecting phosphorylated retinoblastoma protein, which plays a crucial role in regulating cell cycle progression, differentiation, and apoptosis. Rb protein is primarily located in the nucleus, where Rb functions as a tumor suppressor by controlling the transition from G1 phase to S phase of the cell cycle. This localization enables Rb to interact with transcription factors and other regulatory proteins that govern cell proliferation. When Rb is phosphorylated at Ser 795, Rb undergoes a conformational change leading to inactivation, thereby releasing repression on target genes that promote cell cycle progression. Dysregulation of Rb, often due to mutations in the retinoblastoma tumor suppressor gene, is implicated in various cancers, including pediatric retinoblastoma, highlighting the importance of monitoring Rb phosphorylation status in cancer research and therapeutic development.

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p-Rb Antibody (B-4) References:

1. Phosphorylated retinoblastoma protein complexes with pp32 and inhibits pp32-mediated apoptosis.  |  Adegbola, O. and Pasternack, GR. 2005. J Biol Chem. 280: 15497-502. PMID: 15716273
2. Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation.  |  Takemura, M., et al. 2008. Biosci Biotechnol Biochem. 72: 630-5. PMID: 18256459
3. The cell cycle regulator phosphorylated retinoblastoma protein is associated with tau pathology in several tauopathies.  |  Stone, JG., et al. 2011. J Neuropathol Exp Neurol. 70: 578-87. PMID: 21666500
4. Discrete phosphorylated retinoblastoma protein isoform expression in mouse tooth development.  |  Zhang, W., et al. 2012. J Mol Histol. 43: 281-8. PMID: 22476877
5. Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats.  |  Liu, W., et al. 2013. J Mol Histol. 44: 147-58. PMID: 23371354
6. Expression of the oncoprotein gankyrin and phosphorylated retinoblastoma protein in human testis and testicular germ cell tumor.  |  Ando, S., et al. 2014. Int J Urol. 21: 992-8. PMID: 24861729
7. Phosphorylated retinoblastoma protein is a potential predictive marker of irinotecan efficacy for colorectal cancer.  |  Ikai, A., et al. 2016. Int J Oncol. 48: 1297-304. PMID: 26783196
8. Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression.  |  Jin, X., et al. 2019. Mol Cell. 73: 22-35.e6. PMID: 30527665
9. SETDB1 Modulates Degradation of Phosphorylated RB and Anticancer Efficacy of CDK4/6 Inhibitors.  |  Huang, Z., et al. 2023. Cancer Res. 83: 875-889. PMID: 36637424
10. Phosphorylated retinoblastoma protein stimulates DNA polymerase alpha.  |  Takemura, M., et al. 1997. Oncogene. 15: 2483-92. PMID: 9395244

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Rb Antibody (B-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Rb Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Rb Antibody (B-4) Specifications

Alternate Product Name: RB1

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 795

Epitope Target: Rb

Epitope Species: human

Product Reactivity/Detection: Ser 795 phosphorylated Rb

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-Rb Antibody (B-4): P: Blocking Peptide, X: TransCruz Reagent

The preferred Rb antibody is Rb Antibody (IF8): sc-102.