p-PKC alpha Antibody (A-11): sc-377565

p-PKC α Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects phosphorylated protein kinase C alpha (p-PKC α) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-PKC α Antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The protein kinase C (PKC) family, to which p-PKC α belongs, is crucial for regulating numerous cellular processes, such as cell growth, differentiation, and apoptosis, making p-PKC α a significant target for understanding various diseases, including cancer. p-PKC α is primarily located in the cytoplasm and can translocate to the plasma membrane upon activation, which is essential for signal transduction pathways. This translocation enables activation of downstream signaling cascades that influence cellular responses to growth factors and hormones. Phosphorylation of Ser 657 modulates p-PKC α activity, allowing participation in critical cellular functions. Understanding p-PKC α localization and activation can provide insights into pathophysiological conditions and potential therapeutic targets.

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p-PKC alpha Antibody (A-11) References:

1. Mammalian TOR controls one of two kinase pathways acting upon nPKCdelta and nPKCepsilon.  |  Parekh, D., et al. 1999. J Biol Chem. 274: 34758-64. PMID: 10574945
2. Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro.  |  Konishi, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 6587-92. PMID: 11381116
3. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
4. Enhanced phosphorylation of caveolar PKC-α limits peptide internalization in lung endothelial cells.  |  Hutchinson, TE., et al. 2012. Mol Cell Biochem. 360: 309-20. PMID: 21948261
5. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
6. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
7. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
8. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
9. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
10. Activation of protein kinase C by tyrosine phosphorylation in response to H2O2.  |  Konishi, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 11233-7. PMID: 9326592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKC alpha Antibody (A-11) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKC alpha Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKC alpha Antibody (A-11) Specifications

Alternate Product Name: PKC alpha

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 657

Epitope Target: PKC α

Epitope Species: human

Product Reactivity/Detection: Ser 657 phosphorylated PKC α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for p-PKC alpha Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PKC alpha antibody is PKC alpha Antibody (H-7): sc-8393.