p-PFK-2/PFKFB2 Antibody (B-3): sc-373806

PFKFB2 Antibody (B-3) is an IgG1 κ mouse monoclonal p-PFKFB2 antibody that detects Ser 483 phosphorylated cardiac-type PFK-2 of mouse, rat and human origin by WB, IP, IF and ELISA. p-PFKFB2 Antibody (B-3) is available as the non-conjugated anti-PFKFB2 antibody form, as well as multiple conjugated forms of anti-p-PFKFB2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Phosphofructose kinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2,6-bisphosphate (F2,6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2,6BP. F2,6BP functions as a potent activator of 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed and they are differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumulation of intracellular F2,6BP. Activation of PFK-2 cardiac (PFKFB2, or PFK-2 car) by insulin results from Ser 466 and Ser 483 phosphorylation and requires a PDK-1-activated protein kinase other than PKB.

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p-PFK-2/PFKFB2 Antibody (B-3) References:

1. An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and the Warburg effect.  |  Chesney, J., et al. 1999. Proc Natl Acad Sci U S A. 96: 3047-52. PMID: 10077634
2. Expression and regulation of 6-phosphofructo-2-kinase/fructose- 2,6-bisphosphatase isozymes in white adipose tissue.  |  Bruni, P., et al. 1999. Eur J Biochem. 259: 756-61. PMID: 10092861
3. Heart 6-phosphofructo-2-kinase activation by insulin results from Ser-466 and Ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase B.  |  Bertrand, L., et al. 1999. J Biol Chem. 274: 30927-33. PMID: 10521487
4. Tissue-specific alternative splicing of rat brain fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase.  |  Watanabe, F. and Furuya, E. 1999. FEBS Lett. 458: 304-8. PMID: 10570929
5. Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during ischaemia.  |  Marsin, AS., et al. 2000. Curr Biol. 10: 1247-55. PMID: 11069105
6. Early post-mortem AMP-activated protein kinase (AMPK) activation leads to phosphofructokinase-2 and -1 (PFK-2 and PFK-1) phosphorylation and the development of pale, soft, and exudative (PSE) conditions in porcine longissimus muscle.  |  Shen, QW., et al. 2006. J Agric Food Chem. 54: 5583-9. PMID: 16848549
7. Hormonal control of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression in rat hepatoma cells.  |  Cifuentes, ME., et al. 1991. J Biol Chem. 266: 1557-63. PMID: 1846360
8. Ribokinase family evolution and the role of conserved residues at the active site of the PfkB subfamily representative, Pfk-2 from Escherichia coli.  |  Cabrera, R., et al. 2010. Arch Biochem Biophys. 502: 23-30. PMID: 20599671
9. Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase.  |  Algaier, J. and Uyeda, K. 1988. Biochem Biophys Res Commun. 153: 328-33. PMID: 2837207
10. RSK Regulates PFK-2 Activity to Promote Metabolic Rewiring in Melanoma.  |  Houles, T., et al. 2018. Cancer Res. 78: 2191-2204. PMID: 29440170
11. Increase of the glycolytic rate in human resting fibroblasts following serum stimulation. The possible role of the fructose-2,6-bisphosphate.  |  Bruni, P., et al. 1983. FEBS Lett. 159: 39-42. PMID: 6223840
12. Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines.  |  Hirata, T., et al. 1998. Biochem Biophys Res Commun. 242: 680-4. PMID: 9464277

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PFK-2/PFKFB2 Antibody (B-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PFK-2/PFKFB2 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PFK-2/PFKFB2 Antibody (B-3) Specifications

Alternate Product Name: PFKFB2

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 483

Epitope Target: PFKFB2

Epitope Species: human

Product Reactivity/Detection: Ser 483 phosphorylated PFKFB2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-PFK-2/PFKFB2 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred PFK-2/PFKFB2 antibody is PFK-2/PFKFB2 Antibody (D-1): sc-377416.