p-NFκB p50 Antibody (A-8): sc-271908

p-NFκB p50 Antibody (A-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects p-NFκB p50 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p-NFκB p50 antibody (A-8) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. p-NFκB p50 plays a crucial role in the regulation of gene expression as a component of the NFκB transcription factor complex, which is essential for mediating immune responses, inflammation, and cell survival. The NFκB complex is typically sequestered in the cytoplasm in an inactive form bound to inhibitors, but upon stimulation by various signals, such as cytokines or growth factors, NFκB translocates to the nucleus where NFκB binds to specific DNA sequences to activate transcription of target genes. The p50 subunit is generated from the processing of the p105 precursor protein, and phosphorylation at serine 337 is a key regulatory mechanism that influences transcriptional activity and interaction with other proteins in the NFκB pathway. Understanding the dynamics of p-NFκB p50 and post-translational modifications is vital for elucidating its role in various pathological conditions, including cancer and autoimmune diseases, making p-NFκB p50 (A-8) monoclonal antibody an invaluable tool for researchers studying these critical biological processes.

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p-NFκB p50 Antibody (A-8) References:

1. Phosphatase activity in the arterial wall after balloon injury: effect of somatostatin analog octreotide.  |  Yamashita, M., et al. 1999. Lab Invest. 79: 935-44. PMID: 10462031
2. Bcl-3 and NFkappaB p50-p50 homodimers act as transcriptional repressors in tolerant CD4+ T cells.  |  Grundström, S., et al. 2004. J Biol Chem. 279: 8460-8. PMID: 14668329
3. The Nuclear Factor-kappa B p50 subunit is involved in flow-induced outward arterial remodeling.  |  van Keulen, JK., et al. 2009. Atherosclerosis. 202: 424-30. PMID: 18617174
4. Nuclear factor kappa B subunit p50 promotes melanoma angiogenesis by upregulating interleukin-6 expression.  |  Karst, AM., et al. 2009. Int J Cancer. 124: 494-501. PMID: 18942706
5. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product.  |  Kieran, M., et al. 1990. Cell. 62: 1007-18. PMID: 2203531
6. Deletion of Nuclear Factor kappa B p50 Subunit Decreases Inflammatory Response and Mildly Protects Neurons from Transient Forebrain Ischemia-induced Damage.  |  Rolova, T., et al. 2016. Aging Dis. 7: 450-65. PMID: 27493832
7. Evaluation of the Chemopreventive Activity of Grape Skin Extract Using Medium-term Oral Carcinogenesis Assay Induced by 4-Nitroquinoline 1-Oxide.  |  DE Moura, CFG., et al. 2019. Anticancer Res. 39: 177-182. PMID: 30591456
8. A Network Pharmacology Approach to Identify Potential Molecular Targets for Cannabidiol's Anti-Inflammatory Activity.  |  Ma, H., et al. 2021. Cannabis Cannabinoid Res. 6: 288-299. PMID: 33998855
9. Andrographolide-based potential anti-inflammatory transcription inhibitors against nuclear factor NF-kappa-B p50 subunit (NF-κB p50): an integrated molecular and quantum mechanical approach.  |  Jain, P. and Sudandiradoss, C. 2023. 3 Biotech. 13: 15. PMID: 36540414
10. Epidermal Growth Factor Receptor Mediates Neuronal Apoptosis After Subarachnoid Hemorrhage in Mice.  |  Nakano, F., et al. 2023. Stroke. 54: 1616-1626. PMID: 37154060

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-NFκB p50 Antibody (A-8) has 56 citations in a variety of scientific publications.

Primary antibodies like Anti-p-NFκB p50 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-NFκB p50 Antibody (A-8) Specifications

Alternate Product Name: NFKB1

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Ser 337

Epitope Target: p-NFκB p50

Epitope Species: human

Product Reactivity/Detection: Ser 337 phosphorylated NFκB p50

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 56 citations in various scientific publications

Available Conjugates/Forms for p-NFκB p50 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred NFκB p50 antibody is NFκB p50 Antibody (E-10): sc-8414.