NFκB p50 Antibody (E-10): sc-8414

NFκB p50 Antibody (E-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects NFκB p50 protein of mouse, rat, and human origin through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). NFκB p50 (E-10) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. NFκB p50 protein plays a crucial role in regulating immune responses and inflammation, acting as a transcription factor that controls the expression of genes involved in these processes. NFκB p50 translocates from the cytoplasm to the nucleus in response to mitogenic signals, which is vital for the activation of immune cells and proper immune function and response to pathogens. NFκB p50 subunit is derived from the precursor protein p105, which contains a regulatory domain that can modulate NFκB p50 activity, highlighting the intricate control mechanisms governing NFκB signaling pathways. Understanding NFκB p50 function is important for elucidating NFκB p50 role in various diseases, including cancer and autoimmune disorders, where dysregulation of this pathway can lead to pathological conditions.

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NFκB p50 Antibody (E-10) References:

1. Duration of nuclear NF-kappaB action regulated by reversible acetylation.  |  Chen Lf,., et al. 2001. Science. 293: 1653-7. PMID: 11533489
2. The ankyrin repeat domains of the NF-kappa B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-kappa B DNA binding.  |  Hatada, EN., et al. 1992. Proc Natl Acad Sci U S A. 89: 2489-93. PMID: 1532257
3. The 65-kDa subunit of human NF-kappa B functions as a potent transcriptional activator and a target for v-Rel-mediated repression.  |  Ballard, DW., et al. 1992. Proc Natl Acad Sci U S A. 89: 1875-9. PMID: 1542686
4. Intramolecular masking of the nuclear location signal and dimerization domain in the precursor for the p50 NF-kappa B subunit.  |  Henkel, T., et al. 1992. Cell. 68: 1121-33. PMID: 1547506
5. NFkappaB1/p50 is not required for tumor necrosis factor-stimulated growth of primary mammary epithelial cells: implications for NFkappaB2/p52 and RelB.  |  Zhang, J., et al. 2007. Endocrinology. 148: 268-78. PMID: 17008396
6. Cloning of an NF-kappa B subunit which stimulates HIV transcription in synergy with p65.  |  Schmid, RM., et al. 1991. Nature. 352: 733-6. PMID: 1876189
7. The biological functions of NF-kappaB1 (p50) and its potential as an anti-cancer target.  |  Yu, Y., et al. 2009. Curr Cancer Drug Targets. 9: 566-71. PMID: 19519322
8. Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha.  |  Meyer, R., et al. 1991. Proc Natl Acad Sci U S A. 88: 966-70. PMID: 1992489
9. Loss of NF-κB p50 function synergistically augments microglial priming in the middle-aged brain.  |  Taetzsch, T., et al. 2019. J Neuroinflammation. 16: 60. PMID: 30871598
10. A novel STAT3/ NFκB p50 axis regulates stromal-KDM2A to promote M2 macrophage-mediated chemoresistance in breast cancer.  |  Chen, JS., et al. 2023. Cancer Cell Int. 23: 237. PMID: 37821959
11. Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-kappa B.  |  Beg, AA., et al. 1995. Nature. 376: 167-70. PMID: 7603567
12. The crystal structure of the IkappaBalpha/NF-kappaB complex reveals mechanisms of NF-kappaB inactivation.  |  Huxford, T., et al. 1998. Cell. 95: 759-70. PMID: 9865694

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NFκB p50 Antibody (E-10) has 371 citations in a variety of scientific publications.

Primary antibodies like Anti-NFκB p50 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NFκB p50 Antibody (E-10) Specifications

Alternate Product Name: NFKB1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 120-239

Epitope Target: NFκB p50

Epitope Species: human

Product Reactivity/Detection: NFκB p50 and p105

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 371 citations in various scientific publications

Available Conjugates/Forms for NFκB p50 Antibody (E-10): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred p-NFκB p50 antibody is p-NFκB p50 Antibody (A-8): sc-271908.