p-NF-H Antibody (RNF405): sc-32730

p-NF-H (RNF405) is a mouse monoclonal IgG2a kappa light chain antibody that detects phosphorylated NF-H in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). anti-p-NF-H antibody (RNF405) is available non-conjugated or conjugated to agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® dyes. Neurofilament-H (NF-H), a member of the intermediate filament family, serves as a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures containing phosphorylation sites for various protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase g. Beyond controlling axon caliber, neurofilaments may influence other cytoskeletal elements like microtubules and Actin filaments. Altered neurofilament phosphorylation or metabolism commonly occurs in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-NF-H Antibody (RNF405) References:

1. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.  |  Nakamura, Y., et al. 1999. FEBS Lett. 455: 83-6. PMID: 10428477
2. Central and systemic endotoxin challenges exacerbate the local inflammatory response and increase neuronal death during chronic neurodegeneration.  |  Cunningham, C., et al. 2005. J Neurosci. 25: 9275-84. PMID: 16207887
3. Heterogeneous inclusions in neurofilament inclusion disease.  |  Uchikado, H., et al. 2006. Neuropathology. 26: 417-21. PMID: 17080718
4. Effects of phosphorylation of the neurofilament L protein on filamentous structures.  |  Hisanaga, S., et al. 1990. Cell Regul. 1: 237-48. PMID: 2100199
5. Involvement of protein kinase C in the regulation of assembly-disassembly of neurofilaments in vitro.  |  Gonda, Y., et al. 1990. Biochem Biophys Res Commun. 167: 1316-25. PMID: 2108674
6. The involvement of neurofilament heavy chain phosphorylation in the maturation and degeneration of rat oocytes.  |  Takahashi, N. and Ishizuka, B. 2012. Endocrinology. 153: 1990-8. PMID: 22315443
7. In vivo phosphorylation of distinct domains of the 70-kilodalton neurofilament subunit involves different protein kinases.  |  Sihag, RK. and Nixon, RA. 1989. J Biol Chem. 264: 457-64. PMID: 2491851
8. Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures.  |  Angelides, KJ., et al. 1989. J Cell Biol. 108: 1495-506. PMID: 2925792
9. The gene encoding the large human neurofilament subunit (NF-H) maps to the q121-q131 region on human chromosome 22.  |  Mattei, MG., et al. 1988. Hum Genet. 80: 293-5. PMID: 3192217
10. Expression of phosphorylated high molecular weight neurofilament protein (NF-H) and vimentin in human developing dorsal root ganglia and spinal cord.  |  Lukás, Z., et al. 1993. Histochemistry. 100: 495-502. PMID: 8163392
11. The heavy neurofilament protein is expressed in regenerating adult but not embryonic mammalian optic fibers in vitro.  |  Bates, CA. and Meyer, RL. 1993. Exp Neurol. 119: 249-57. PMID: 8432362
12. Abnormal distribution of neurofilament L in neurons with Alzheimer's disease.  |  Nakamura, Y., et al. 1997. Neurosci Lett. 225: 201-4. PMID: 9147405

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-NF-H Antibody (RNF405) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-NF-H Antibody (RNF405) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-NF-H Antibody (RNF405) Specifications

Alternate Product Name: NF-H

Product Clone Name: RNF405

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: phosphorylated NF-H protein

Epitope Target: p-NF-H

Epitope Species: bovine

Product Reactivity/Detection: phosphorylated NF-H

Species Reactivity/Detection: mouse, rat, human, Xenopus, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-NF-H Antibody (RNF405): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NF-H antibody is NF-H Antibody (RNF402): sc-32729.