Western blot analysis of IκB-α phosphorylation in untreated (A,C) and TNF-α induced (B,D) HeLa cells. Antibodies tested include p-IκB-α Antibody (B-9): sc-8404 (A,B) and IκB-α (H-4): sc-1643 (C,D).
p-NFKBIA/IkB alpha Antibody (B-9): sc-8404
- p-NFKBIA/IkB alpha Antibody (B-9) is a mouse monoclonal IgG2b κ, cited in 524 publications, provided at 200 µg/ml
- Anti-p-NFKBIA/IkB alpha Antibody (B-9) is recommended for detection of IκB-α phosphorylated at Ser 32 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-p-NFKBIA/IkB alpha Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-NFKBIA/IkB alpha Antibody (B-9) for WB and IHC(P) applications. These reagents are now offered in bundles with p-NFKBIA/IkB alpha Antibody (B-9) (see ordering information below).
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Research Information
p-IκB-α Antibody (B-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects IκB-α phosphorylated at Ser 32 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p-IκB-α (B-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IκB-α plays a crucial role in regulating the NF-κB signaling pathway, which controls immune responses, inflammation, and cell survival. By sequestering NF-κB dimers in the cytoplasm, IκB-α prevents their translocation to the nucleus, thereby inhibiting the transcription of target genes involved in these processes. Phosphorylation of IκB-α at Ser 32 marks IκB-α for degradation, allowing NF-κB to enter the nucleus and activate gene expression. Understanding IκB-α phosphorylation dynamics is vital for studying various diseases, including cancer and autoimmune disorders, where NF-κB signaling is often dysregulated.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-NFKBIA/IkB alpha Antibody (B-9) References:
- I kappa B gamma, a 70 kd protein identical to the C-terminal half of p110 NF-kappa B: a new member of the I kappa B family. | Inoue, J., et al. 1992. Cell. 68: 1109-20. PMID: 1339305
- Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. | Haskill, S., et al. 1991. Cell. 65: 1281-9. PMID: 1829648
- Rel-associated pp40: an inhibitor of the rel family of transcription factors. | Davis, N., et al. 1991. Science. 253: 1268-71. PMID: 1891714
- The rel-associated pp40 protein prevents DNA binding of Rel and NF-kappa B: relationship with I kappa B beta and regulation by phosphorylation. | Kerr, LD., et al. 1991. Genes Dev. 5: 1464-76. PMID: 1907941
- Activation in vitro of NF-kappa B by phosphorylation of its inhibitor I kappa B. | Ghosh, S. and Baltimore, D. 1990. Nature. 344: 678-82. PMID: 2157987
- Incorporation of Phosphorylated Tyrosine into Proteins: In Vitro Translation and Study of Phosphorylated IκB-α and Its Interaction with NF-κB. | Chen, S., et al. 2017. J Am Chem Soc. 139: 14098-14108. PMID: 28898075
- Nur77 attenuates inflammatory responses and oxidative stress by inhibiting phosphorylated IκB-α in Parkinson's disease cell model. | Yan, J., et al. 2020. Aging (Albany NY). 12: 8107-8119. PMID: 32401747
- I kappa B-beta regulates the persistent response in a biphasic activation of NF-kappa B. | Thompson, JE., et al. 1995. Cell. 80: 573-82. PMID: 7867065
- I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity. | Whiteside, ST., et al. 1997. EMBO J. 16: 1413-26. PMID: 9135156
- Cloning and functional characterization of mouse IkappaBepsilon. | Simeonidis, S., et al. 1997. Proc Natl Acad Sci U S A. 94: 14372-7. PMID: 9405619
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-NFKBIA/IkB alpha Antibody (B-9) | sc-8404 | 200 µg/ml | $322.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-528222 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-520548 | 200 µg Ab, 40 µg BP | $361.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) AC | sc-8404 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) HRP | sc-8404 HRP | 200 µg/ml | $322.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) FITC | sc-8404 FITC | 200 µg/ml | $336.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) PE | sc-8404 PE | 200 µg/ml | $349.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 488 | sc-8404 AF488 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 546 | sc-8404 AF546 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 594 | sc-8404 AF594 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 647 | sc-8404 AF647 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 680 | sc-8404 AF680 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha Antibody (B-9) Alexa Fluor® 790 | sc-8404 AF790 | 200 µg/ml | $364.00 | |||
p-NFKBIA/IkB alpha (B-9) Neutralizing Peptide | sc-8404 P | 100 µg/0.5 ml | $69.00 |