p-HSP 27 Antibody (B-3) recommended for detection of Ser 82 phosphorylated HSP 27 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p-HSP 27 Antibody (B-3): sc-166693

p-HSP 27 Antibody (B-3) is rated 4.4 out of 5 by 10.
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Datasheets

    • p-HSP 27 Antibody (B-3) is a mouse monoclonal IgG2b κ, cited in 7 publications, provided at 200 µg/ml
    • Anti-p-HSP 27 Antibody (B-3) is recommended for detection of Ser 82 phosphorylated HSP 27 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-HSP 27 Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-HSP 27 (B-3): sc-166693.

What application is the blocking peptide sc-166693 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from excellent antibody for western blotting Very clear and specific band in western blotting of C2C12, 16-HBE, CFBE cells.
Date published: 2018-08-29
Rated 5 out of 5 by from Muy buen anticuerpo para WB Funciona muy bien en extractos celulares de células AGS
Date published: 2018-01-11
Rated 5 out of 5 by from great antibody for western blot The batch of antibody I have worked well on HeLa and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 1'000 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 4 out of 5 by from Good antibody There is a very perfect band in my sample, while in some samples, there is another very closed band.
Date published: 2017-12-04
Rated 3 out of 5 by from Pity awful bind for my cells. may be it is a lower expression in my cells. thanks for the antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from quite clear band for wb of rat protein 1:1000 diluted for wb of rat protein ,the band is so clear!
Date published: 2017-07-13
Rated 5 out of 5 by from Very potent and highly specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of target ~30 kDa HSP27 in human HeLa cells treated for 2 hours with anisomycin on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-04
Rated 4 out of 5 by from Antibody worked well in IF at 1:50 dilution Antibody worked well in IF at 1:50 dilution.
Date published: 2015-07-24
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