p-HSP 27 Antibody (B-3): sc-166693

p-HSP 27 Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 82 phosphorylated HSP 27 (also known as HSPB1) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-p-HSP 27 antibody (B-3) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. HSP 27 plays a crucial role in cellular stress responses, functioning as a molecular chaperone that helps prevent protein aggregation and assists in refolding denatured proteins. HSP 27′s ability to translocate from cytoplasm to nucleus under stress conditions is vital for protecting cells from apoptosis and promoting cell survival. Phosphorylation of HSP 27 at Serine residues, particularly Ser 15, Ser 78, and Ser 82, is essential for chaperone activity and is regulated by various signaling pathways, including those activated by growth factors and heat shock. This post-translational modification enhances protective functions and modulates interactions with other proteins, thereby influencing numerous cellular processes.

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p-HSP 27 Antibody (B-3) References:

1. Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins.  |  Stokoe, D., et al. 1992. FEBS Lett. 313: 307-13. PMID: 1332886
2. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II.  |  Landry, J., et al. 1992. J Biol Chem. 267: 794-803. PMID: 1730670
3. Hsp-27 induction requires POU4F2/Brn-3b TF in doxorubicin-treated breast cancer cells, whereas phosphorylation alters its cellular localisation following drug treatment.  |  Fujita, R., et al. 2011. Cell Stress Chaperones. 16: 427-39. PMID: 21279488
4. Heat shock proteins in the brain: role of Hsp70, Hsp 27, and HO-1 (Hsp32) and their therapeutic potential.  |  Sharp, FR., et al. 2013. Transl Stroke Res. 4: 685-92. PMID: 24323422
5. Palytoxin induces dissociation of HSP 27 oligomers through a p38 protein kinase pathway.  |  Berni, C., et al. 2015. Chem Res Toxicol. 28: 752-64. PMID: 25710824
6. Hsp-27 and NF-κB pathway is associated with AR/AR-V7 expression in prostate cancer cells.  |  Kiliccioglu, I., et al. 2019. Gene. 697: 138-143. PMID: 30807779
7. Cytokines and growth factors induce HSP27 phosphorylation in human astrocytes.  |  Satoh, J. and Kim, SU. 1995. J Neuropathol Exp Neurol. 54: 504-12. PMID: 7541446
8. Tumor necrosis factor-alpha induces changes in the phosphorylation, cellular localization, and oligomerization of human hsp27, a stress protein that confers cellular resistance to this cytokine.  |  Mehlen, P., et al. 1995. J Cell Biochem. 58: 248-59. PMID: 7673331
9. Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of Hsp27.  |  Freshney, NW., et al. 1994. Cell. 78: 1039-49. PMID: 7923354
10. Biological and clinical implications of heat shock protein 27,000 (Hsp27): a review.  |  Ciocca, DR., et al. 1993. J Natl Cancer Inst. 85: 1558-70. PMID: 8411230

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-HSP 27 Antibody (B-3) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-p-HSP 27 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-HSP 27 Antibody (B-3) Specifications

Alternate Product Name: HSPB1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 82

Epitope Target: HSP 27

Epitope Species: human

Product Reactivity/Detection: Ser 82 phosphorylated HSP 27

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for p-HSP 27 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred HSP 27 antibody is HSP 27 Antibody (F-4): sc-13132.