p-Histone H3 Antibody (C-2): sc-374669

p-Histone H3 Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 28 phosphorylated Histone H3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Histone H3 (C-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Histone H3 plays a crucial role in the structural organization of chromatin, as Histone H3 is a core component of the nucleosome, which consists of an octamer of histones that wraps around approximately 200 base pairs of DNA. This structural arrangement is vital for the regulation of gene expression, as phosphorylation of Histone H3 at specific sites, such as Ser 28, is associated with chromatin remodeling during cell division, particularly in prophase. The phosphorylation events at these sites facilitate the dynamic changes in chromatin structure that are necessary for proper mitotic progression and gene regulation. Anti-p-Histone H3 antibody (C-2) enables researchers to gain insights into the phosphorylation status of Histone H3, thereby elucidating Histone H3′s role in chromatin dynamics and cellular processes.

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p-Histone H3 Antibody (C-2) References:

1. Meiotic events at the centromeric heterochromatin: histone H3 phosphorylation, topoisomerase II alpha localization and chromosome condensation.  |  Cobb, J., et al. 1999. Chromosoma. 108: 412-25. PMID: 10654080
2. ERKs and p38 kinases mediate ultraviolet B-induced phosphorylation of histone H3 at serine 10.  |  Zhong, SP., et al. 2000. J Biol Chem. 275: 20980-4. PMID: 10806218
3. Expression of Aurora-B kinase and phosphorylated histone H3 in hepatocellular carcinoma.  |  Sistayanarain, A., et al. 2006. Anticancer Res. 26: 3585-93. PMID: 17094487
4. Dynamic distribution of Thr3-phosphorylated histone H3 in CHO cells in mitosis.  |  Zhou, H., et al. 2006. Folia Biol (Praha). 52: 156-60. PMID: 17116287
5. Phosphorylated histone H3, Ki-67, p21, fatty acid synthase, and cleaved caspase-3 expression in benign and atypical granular cell tumors.  |  Kapur, P., et al. 2007. Arch Pathol Lab Med. 131: 57-64. PMID: 17227124
6. Modulation of 14-3-3 interaction with phosphorylated histone H3 by combinatorial modification patterns.  |  Winter, S., et al. 2008. Cell Cycle. 7: 1336-42. PMID: 18418070
7. Mitosis-specific histone H3 phosphorylation in vitro in nucleosome structures.  |  Shibata, K., et al. 1990. Eur J Biochem. 192: 87-93. PMID: 2401299
8. The H1 and core histone subtypes: differential gene expression and varied primary structures.  |  Doenecke, D., et al. 1988. Adv Enzyme Regul. 27: 107-20. PMID: 3250226
9. Improving fine needle aspiration to predict the tumor biological aggressiveness in pancreatic neuroendocrine tumors using Ki-67 proliferation index, phosphorylated histone H3 (PHH3), and BCL-2.  |  Zhao, CL., et al. 2023. Ann Diagn Pathol. 65: 152149. PMID: 37119647
10. Histone H1.  |  Wolffe, AP. 1997. Int J Biochem Cell Biol. 29: 1463-6. PMID: 9570139

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Histone H3 Antibody (C-2) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Histone H3 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Histone H3 Antibody (C-2) Specifications

Alternate Product Name: phospho Histone H3

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 28

Epitope Target: Histone H3

Epitope Species: human

Product Reactivity/Detection: Ser 28 phosphorylated Histone H3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for p-Histone H3 Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Ac-Histone H3 antibody is Ac-Histone H3 Antibody (AH3-120): sc-56616.