p-Ezrin Antibody (G-7): sc-166858

p-Ezrin Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tyr 146 phosphorylated Ezrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Ezrin (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ezrin, a key microvillar core protein, plays a crucial role in linking the plasma membrane to the actin cytoskeleton, which is essential for maintaining cell shape and facilitating cell adhesion. Phosphorylation of Ezrin at Tyr 146 is particularly significant as this site is located within a conserved region shared by the Protein 4.1-Talin-Ezrin family, which includes Radixin and Moesin. This phosphorylation event is critical for Ezrin activation, influencing cellular morphology and adhesion properties, especially in response to extracellular signals like epidermal growth factor. Understanding Ezrin phosphorylation dynamics can provide insights into cellular processes such as migration, proliferation, and tissue architecture maintenance, making anti-p-Ezrin antibody (G-7) an invaluable tool for researchers studying these fundamental biological mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Ezrin Antibody (G-7) References:

1. Tyrosine phosphorylation and cellular redistribution of ezrin in MDCK cells treated with pervanadate.  |  Wu, YX., et al. 2000. J Cell Biochem. 79: 311-21. PMID: 10967558
2. Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells.  |  Egerton, M., et al. 1992. J Immunol. 149: 1847-52. PMID: 1381389
3. Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin.  |  Krieg, J. and Hunter, T. 1992. J Biol Chem. 267: 19258-65. PMID: 1382070
4. Moesin: a member of the protein 4.1-talin-ezrin family of proteins.  |  Lankes, WT. and Furthmayr, H. 1991. Proc Natl Acad Sci U S A. 88: 8297-301. PMID: 1924289
5. Phosphorylated ezrin is located in the nucleus of the osteosarcoma cell.  |  Di Cristofano, C., et al. 2010. Mod Pathol. 23: 1012-20. PMID: 20348881
6. Differential ezrin and phosphorylated ezrin expression profiles between pancreatic intraepithelial neoplasia, intraductal papillary mucinous neoplasm, and invasive ductal carcinoma of the pancreas.  |  Oda, Y., et al. 2013. Hum Pathol. 44: 1487-98. PMID: 23465281
7. Prognostic implications of ezrin and phosphorylated ezrin expression in non-small cell lung cancer.  |  Jin, T., et al. 2014. BMC Cancer. 14: 191. PMID: 24629131
8. Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver.  |  Xue, Y., et al. 2020. Am J Pathol. 190: 1427-1437. PMID: 32289287
9. Expression of ezrin protein and phosphorylated ezrin in pelvic endometriotic lesions.  |  Peloggia, A., et al. 2022. Clinics (Sao Paulo). 77: 100074. PMID: 35793608
10. Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells.  |  Hunter, T. and Cooper, JA. 1981. Cell. 24: 741-52. PMID: 6166387

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Ezrin Antibody (G-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Ezrin Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Ezrin Antibody (G-7) Specifications

Alternate Product Name: p-Ezrin

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 146

Epitope Target: Ezrin

Epitope Species: human

Product Reactivity/Detection: Tyr 146 phosphorylated Ezrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-Ezrin Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Ezrin antibody is Ezrin Antibody (3C12): sc-58758.