Ezrin Antibody (3C12): sc-58758
- Ezrin Antibody (3C12) is a mouse monoclonal IgG1 κ Ezrin antibody, cited in 71 publications, provided at 200 µg/ml
- raised against amino acids 362-585 of Ezrin of human origin
- Ezrin Antibody (3C12) is recommended for detection of Ezrin of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
- Anti-Ezrin Antibody (3C12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ezrin Antibody (3C12) for WB and IHC(P) applications. These reagents are now offered in bundles with Ezrin Antibody (3C12) (see ordering information below).
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Ezrin Antibody (3C12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ezrin protein of mouse, rat, human, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Ezrin antibody (3C12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ezrin plays a crucial role in linking the cytoskeleton to the plasma membrane, which is essential for maintaining cell shape and facilitating cellular signaling. Ezrin is primarily located just beneath the plasma membrane, where Ezrin interacts with other cytoskeletal and membrane proteins, thereby influencing processes such as cell adhesion, migration, and morphology. Notably, Ezrin acts as a major cytoplasmic substrate for various protein-tyrosine kinases, including the epidermal growth factor receptor, and functions as an A-kinase anchoring protein, designated AKAP78. Ezrin′s interaction with Moesin and Radixin, which share over 70% homology, highlights the importance of this protein family in cellular dynamics, as these proteins exhibit distinct receptor-specific patterns of phosphorylation despite their similarities. This specificity is vital for the regulation of various signaling pathways, making Ezrin a key player in cellular responses to external stimuli.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ezrin Antibody (3C12) References:
- A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites. | Sato, N., et al. 1992. J Cell Sci. 103 (Pt 1): 131-43. PMID: 1429901
- Moesin: a member of the protein 4.1-talin-ezrin family of proteins. | Lankes, WT. and Furthmayr, H. 1991. Proc Natl Acad Sci U S A. 88: 8297-301. PMID: 1924289
- cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. | Gould, KL., et al. 1989. EMBO J. 8: 4133-42. PMID: 2591371
- Perspectives for Ezrin and Radixin in Astrocytes: Kinases, Functions and Pathology. | Derouiche, A. and Geiger, KD. 2019. Int J Mol Sci. 20: PMID: 31382374
- Membrane Tension and the Role of Ezrin During Phagocytosis. | Roberts, RE., et al. 2020. Adv Exp Med Biol. 1246: 83-102. PMID: 32399827
- Role of Ezrin in Asthma-Related Airway Inflammation and Remodeling. | Zhao, S., et al. 2022. Mediators Inflamm. 2022: 6255012. PMID: 36530558
- Ezrin, radixin, and moesin are dispensable for macrophage migration and cellular cortex mechanics. | Verdys, P., et al. 2024. EMBO J. 43: 4822-4845. PMID: 39026000
- ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons. | Tsukita, S., et al. 1994. J Cell Biol. 126: 391-401. PMID: 7518464
- Ezrin has properties to self-associate at the plasma membrane. | Andréoli, C., et al. 1994. J Cell Sci. 107 (Pt 9): 2509-21. PMID: 7844168
- Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker. | Algrain, M., et al. 1993. J Cell Biol. 120: 129-39. PMID: 8416983
- The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation. | Fazioli, F., et al. 1993. Oncogene. 8: 1335-45. PMID: 8479753
- Ezrin is a cyclic AMP-dependent protein kinase anchoring protein. | Dransfield, DT., et al. 1997. EMBO J. 16: 35-43. PMID: 9009265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ezrin Antibody (3C12) | sc-58758 | 200 µg/ml | $322.00 | |||
Ezrin Antibody (3C12): m-IgG Fc BP-HRP Bundle | sc-528629 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ezrin Antibody (3C12): m-IgGκ BP-HRP Bundle | sc-521059 | 200 µg Ab, 40 µg BP | $361.00 | |||
Ezrin Antibody (3C12): m-IgG1 BP-HRP Bundle | sc-543039 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ezrin Antibody (3C12) AC | sc-58758 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ezrin Antibody (3C12) HRP | sc-58758 HRP | 200 µg/ml | $322.00 | |||
Ezrin Antibody (3C12) FITC | sc-58758 FITC | 200 µg/ml | $336.00 | |||
Ezrin Antibody (3C12) PE | sc-58758 PE | 200 µg/ml | $349.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 488 | sc-58758 AF488 | 200 µg/ml | $364.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 546 | sc-58758 AF546 | 200 µg/ml | $364.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 594 | sc-58758 AF594 | 200 µg/ml | $364.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 647 | sc-58758 AF647 | 200 µg/ml | $364.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 680 | sc-58758 AF680 | 200 µg/ml | $364.00 | |||
Ezrin Antibody (3C12) Alexa Fluor® 790 | sc-58758 AF790 | 200 µg/ml | $364.00 |