Ezrin Antibody (3C12): sc-58758

Ezrin Antibody (3C12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ezrin protein of mouse, rat, human, and bovine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Ezrin antibody (3C12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ezrin plays a crucial role in linking the cytoskeleton to the plasma membrane, which is essential for maintaining cell shape and facilitating cellular signaling. Ezrin is primarily located just beneath the plasma membrane, where Ezrin interacts with other cytoskeletal and membrane proteins, thereby influencing processes such as cell adhesion, migration, and morphology. Notably, Ezrin acts as a major cytoplasmic substrate for various protein-tyrosine kinases, including the epidermal growth factor receptor, and functions as an A-kinase anchoring protein, designated AKAP78. Ezrin′s interaction with Moesin and Radixin, which share over 70% homology, highlights the importance of this protein family in cellular dynamics, as these proteins exhibit distinct receptor-specific patterns of phosphorylation despite their similarities. This specificity is vital for the regulation of various signaling pathways, making Ezrin a key player in cellular responses to external stimuli.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ezrin Antibody (3C12) References:

1. A gene family consisting of ezrin, radixin and moesin. Its specific localization at actin filament/plasma membrane association sites.  |  Sato, N., et al. 1992. J Cell Sci. 103 (Pt 1): 131-43. PMID: 1429901
2. Moesin: a member of the protein 4.1-talin-ezrin family of proteins.  |  Lankes, WT. and Furthmayr, H. 1991. Proc Natl Acad Sci U S A. 88: 8297-301. PMID: 1924289
3. cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1.  |  Gould, KL., et al. 1989. EMBO J. 8: 4133-42. PMID: 2591371
4. Perspectives for Ezrin and Radixin in Astrocytes: Kinases, Functions and Pathology.  |  Derouiche, A. and Geiger, KD. 2019. Int J Mol Sci. 20: PMID: 31382374
5. Membrane Tension and the Role of Ezrin During Phagocytosis.  |  Roberts, RE., et al. 2020. Adv Exp Med Biol. 1246: 83-102. PMID: 32399827
6. Role of Ezrin in Asthma-Related Airway Inflammation and Remodeling.  |  Zhao, S., et al. 2022. Mediators Inflamm. 2022: 6255012. PMID: 36530558
7. Ezrin, radixin, and moesin are dispensable for macrophage migration and cellular cortex mechanics.  |  Verdys, P., et al. 2024. EMBO J. 43: 4822-4845. PMID: 39026000
8. ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons.  |  Tsukita, S., et al. 1994. J Cell Biol. 126: 391-401. PMID: 7518464
9. Ezrin has properties to self-associate at the plasma membrane.  |  Andréoli, C., et al. 1994. J Cell Sci. 107 (Pt 9): 2509-21. PMID: 7844168
10. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker.  |  Algrain, M., et al. 1993. J Cell Biol. 120: 129-39. PMID: 8416983
11. The ezrin-like family of tyrosine kinase substrates: receptor-specific pattern of tyrosine phosphorylation and relationship to malignant transformation.  |  Fazioli, F., et al. 1993. Oncogene. 8: 1335-45. PMID: 8479753
12. Ezrin is a cyclic AMP-dependent protein kinase anchoring protein.  |  Dransfield, DT., et al. 1997. EMBO J. 16: 35-43. PMID: 9009265

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ezrin Antibody (3C12) has 71 citations in a variety of scientific publications.

Primary antibodies like Anti-Ezrin Antibody (3C12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ezrin Antibody (3C12) Specifications

Alternate Product Name: Ezrin

Product Clone Name: 3C12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 362-585

Epitope Target: Ezrin

Epitope Species: human

Product Reactivity/Detection: Ezrin

Species Reactivity/Detection: mouse, rat, human, bovine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 71 citations in various scientific publications

Available Conjugates/Forms for Ezrin Antibody (3C12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin