p-eEF2K Antibody (C-4): sc-377562
- p-eEF2K Antibody (C-4) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids Ser 366 Ser 366 of eEF2K of human origin
- recommended for detection of Ser 366 phosphorylated eEF2K of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine and canine
- m-IgG2b BP-HRP is the preferred secondary detection reagent for p-eEF2K Antibody (C-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-eEF2K Antibody (C-4) (see ordering information below).
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p-eEF2K Antibody (C-4) is a mouse monoclonal IgG2b antibody that detects eukaryotic elongation-factor-2 kinase (eEF2K) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eEF2K antibody (C-4) plays a crucial role in understanding protein synthesis regulation, as eEF2K is a key kinase that modulates translation elongation by phosphorylating and inactivating eEF2. This regulation is particularly important in cellular responses to stress and growth signals, as eEF2K activity is tightly controlled by calcium and calmodulin, which are essential for autophosphorylation on serine and threonine residues. The phosphorylation of eEF2K at specific sites, such as Ser 359 and Ser 366, is influenced by various kinases, including SAPK4 and p70 S6 kinase, and is critical for modulating translation rates under different physiological conditions. Understanding eEF2K phosphorylation dynamics can provide insights into cellular growth, differentiation, and protein synthesis regulation, making eEF2K monoclonal antibody (C-4) an invaluable research tool.
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p-eEF2K Antibody (C-4) References:
- Thermal injury activates the eEF2K-dependent eEF2 pathway in pediatric patients. | Song, J., et al. 2012. JPEN J Parenter Enteral Nutr. 36: 596-602. PMID: 22269896
- Impaired associative taste learning and abnormal brain activation in kinase-defective eEF2K mice. | Gildish, I., et al. 2012. Learn Mem. 19: 116-25. PMID: 22366775
- Pharmacological eEF2K activation promotes cell death and inhibits cancer progression. | De Gassart, A., et al. 2016. EMBO Rep. 17: 1471-1484. PMID: 27572820
- SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy. | Pires Da Silva, J., et al. 2020. Cells. 9: PMID: 32059483
- Bicuculline regulated protein synthesis is dependent on Homer1 and promotes its interaction with eEF2K through mTORC1-dependent phosphorylation. | Gladulich, LFH., et al. 2021. J Neurochem. 157: 1086-1101. PMID: 32892352
- Rpl24Bst mutation suppresses colorectal cancer by promoting eEF2 phosphorylation via eEF2K. | Knight, JR., et al. 2021. Elife. 10: PMID: 34895463
- In silico, synthesis and anticancer evaluation of benzamide tryptamine derivatives as novel eEF2K inhibitors. | Liu, Z., et al. 2022. Bioorg Med Chem Lett. 67: 128759. PMID: 35483594
- Homozygous knockout of eEF2K alleviates cognitive deficits in APP/PS1 Alzheimer's disease model mice independent of brain amyloid β pathology. | Kasica, NP., et al. 2022. Front Aging Neurosci. 14: 959326. PMID: 36158543
- A functional eEF2K-eEF2 pathway in the NAc is critical for the expression of cocaine-induced psychomotor sensitisation and conditioned place preference. | Beiser, T., et al. 2022. Transl Psychiatry. 12: 460. PMID: 36319619
- The alphavirus nonstructural protein 2 NTPase induces a host translational shut-off through phosphorylation of eEF2 via cAMP-PKA-eEF2K signaling. | Treffers, EE., et al. 2023. PLoS Pathog. 19: e1011179. PMID: 36848386
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-eEF2K Antibody (C-4) | sc-377562 | 200 µg/ml | $322.00 | |||
p-eEF2K Antibody (C-4): m-IgG2b BP-HRP Bundle | sc-548614 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-eEF2K (C-4) Neutralizing Peptide | sc-377562 P | 100 µg/0.5 ml | $69.00 |