p-BRCA1 Antibody (G-4): sc-166793
- p-BRCA1 Antibody (G-4) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids Ser 988 of BRCA1 of human origin
- p-BRCA1 Antibody (G-4) is recommended for detection of Ser 988 phosphorylated BRCA1 of human origin by WB, IP, IF and ELISA
- Anti-p-BRCA1 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-BRCA1 Antibody (G-4) for WB applications. These reagents are now offered in bundles with p-BRCA1 Antibody (G-4) (see ordering information below).
p-BRCA1 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 988 phosphorylated BRCA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-BRCA1 (G-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. BRCA1 plays a crucial role in maintaining genomic stability and is essential for DNA double-strand break repair through homologous recombination. BRCA1 function is particularly important in cancer biology, as mutations or dysregulation can lead to increased susceptibility to breast and ovarian cancers. Phosphorylation of BRCA1 at Serine 988 serves as a key regulatory mechanism that influences activity and interactions with other proteins involved in DNA damage response. This phosphorylation event enables BRCA1 release from Chk2, allowing effective participation in repair processes and restoration of cell survival following DNA damage. BRCA1 undergoes various post-translational modifications that further modulate function and interactions, highlighting complex regulatory mechanisms in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-BRCA1 Antibody (G-4) References:
- BRCA1 is phosphorylated at serine 1497 in vivo at a cyclin-dependent kinase 2 phosphorylation site. | Ruffner, H., et al. 1999. Mol Cell Biol. 19: 4843-54. PMID: 10373534
- Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells. | Altiok, S., et al. 1999. J Biol Chem. 274: 32274-8. PMID: 10542266
- Requirement of ATM-dependent phosphorylation of brca1 in the DNA damage response to double-strand breaks. | Cortez, D., et al. 1999. Science. 286: 1162-6. PMID: 10550055
- hCds1-mediated phosphorylation of BRCA1 regulates the DNA damage response. | Lee, JS., et al. 2000. Nature. 404: 201-4. PMID: 10724175
- Role for ATM in DNA damage-induced phosphorylation of BRCA1. | Gatei, M., et al. 2000. Cancer Res. 60: 3299-304. PMID: 10866324
- Phosphorylated BRCA1 is predominantly located in the nucleus and mitochondria. | Coene, ED., et al. 2005. Mol Biol Cell. 16: 997-1010. PMID: 15591126
- BRCA1/P53: Two strengths in cancer chemoprevention. | Raimundo, L., et al. 2020. Biochim Biophys Acta Rev Cancer. 1873: 188339. PMID: 31917206
- Mechanisms of BRCA1-BARD1 nucleosome recognition and ubiquitylation. | Hu, Q., et al. 2021. Nature. 596: 438-443. PMID: 34321665
- Preneoplastic stromal cells promote BRCA1-mediated breast tumorigenesis. | Nee, K., et al. 2023. Nat Genet. 55: 595-606. PMID: 36914836
- BRCA1-Mediated Dual Regulation of Ferroptosis Exposes a Vulnerability to GPX4 and PARP Co-Inhibition in BRCA1-Deficient Cancers. | Lei, G., et al. 2024. Cancer Discov. 14: 1476-1495. PMID: 38552003
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-BRCA1 Antibody (G-4) | sc-166793 | 200 µg/ml | $322.00 | |||
p-BRCA1 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-529052 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-BRCA1 Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-521746 | 200 µg Ab, 40 µg BP | $361.00 | |||
p-BRCA1 Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-543293 | 200 µg Ab; 20 µg BP | $361.00 | |||
p-BRCA1 Antibody (G-4) AC | sc-166793 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-BRCA1 Antibody (G-4) HRP | sc-166793 HRP | 200 µg/ml | $322.00 | |||
p-BRCA1 Antibody (G-4) FITC | sc-166793 FITC | 200 µg/ml | $336.00 | |||
p-BRCA1 Antibody (G-4) PE | sc-166793 PE | 200 µg/ml | $349.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 488 | sc-166793 AF488 | 200 µg/ml | $364.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 546 | sc-166793 AF546 | 200 µg/ml | $364.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 594 | sc-166793 AF594 | 200 µg/ml | $364.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 647 | sc-166793 AF647 | 200 µg/ml | $364.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 680 | sc-166793 AF680 | 200 µg/ml | $364.00 | |||
p-BRCA1 Antibody (G-4) Alexa Fluor® 790 | sc-166793 AF790 | 200 µg/ml | $364.00 | |||
p-BRCA1 (G-4) Neutralizing Peptide | sc-166793 P | 100 µg/0.5 ml | $69.00 |