p-ARK-1 Antibody (95.Thr 288): sc-293126

p-ARK-1 Antibody (95.Thr 288) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Thr 288 phosphorylated ARK-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-ARK-1 antibody (95.Thr 288) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. ARK-1, or aurora related kinase-1, plays a crucial role in cell division by regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 is primarily located in the nucleus and associates with key components of the mitotic machinery, such as the centrosome and spindle poles, which are essential for accurate chromosome alignment and segregation. Proper functioning of ARK-1 is vital for maintaining genomic stability, as errors in these processes can lead to aneuploidy and contribute to tumorigenesis. Notably, ARK-1 is subject to degradation via the ubiquitin-proteasome pathway after the G2/M phase, which is critical for timely progression of the cell cycle. Additionally, ARK-2, a closely related kinase, exhibits distinct expression patterns and functions, highlighting the complexity of regulatory mechanisms governing mitosis. Phosphorylation of ARK-2 on Threonine 288 further underscores the importance of post-translational modifications in modulating the activity and interactions of these kinases during cell cycle progression.

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p-ARK-1 Antibody (95.Thr 288) References:

1. Cdc20 associates with the kinase aurora2/Aik.  |  Farruggio, DC., et al. 1999. Proc Natl Acad Sci U S A. 96: 7306-11. PMID: 10377410
2. Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases.  |  Giet, R. and Prigent, C. 1999. J Cell Sci. 112 (Pt 21): 3591-601. PMID: 10523496
3. Degradation of human Aurora2 protein kinase by the anaphase-promoting complex-ubiquitin-proteasome pathway.  |  Honda, K., et al. 2000. Oncogene. 19: 2812-9. PMID: 10851084
4. Targeting AURKA in Cancer: molecular mechanisms and opportunities for Cancer therapy.  |  Du, R., et al. 2021. Mol Cancer. 20: 15. PMID: 33451333
5. Secretory autophagy-induced bladder tumour-derived extracellular vesicle secretion promotes angiogenesis by activating the TPX2-mediated phosphorylation of the AURKA-PI3K-AKT axis.  |  Li, X., et al. 2021. Cancer Lett. 523: 10-28. PMID: 34597712
6. AURORA KINASE A and related downstream molecules: A potential network for cancer therapy.  |  Fatma, H. and Siddique, HR. 2023. Adv Protein Chem Struct Biol. 134: 115-145. PMID: 36858732
7. Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis.  |  Zhang, W., et al. 2024. Nat Struct Mol Biol. 31: 219-231. PMID: 38177680
8. Involvement of Endolysosomes and Aurora Kinase A in the Regulation of Amyloid β Protein Levels in Neurons.  |  Afghah, Z., et al. 2024. Int J Mol Sci. 25: PMID: 38892390
9. cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2.  |  Shindo, M., et al. 1998. Biochem Biophys Res Commun. 244: 285-92. PMID: 9514916
10. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers.  |  Bischoff, JR., et al. 1998. EMBO J. 17: 3052-65. PMID: 9606188
11. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation.  |  Zhou, H., et al. 1998. Nat Genet. 20: 189-93. PMID: 9771714
12. Identification and characterization of STK12/Aik2: a human gene related to aurora of Drosophila and yeast IPL1.  |  Kimura, M., et al. 1998. Cytogenet Cell Genet. 82: 147-52. PMID: 9858806

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-ARK-1 Antibody (95.Thr 288) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-p-ARK-1 Antibody (95.Thr 288) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-ARK-1 Antibody (95.Thr 288) Specifications

Alternate Product Name: ARK-1

Product Clone Name: 95.Thr 288

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 288

Epitope Target: ARK-1

Epitope Species: human

Product Reactivity/Detection: Thr 288 phosphorylated ARK-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for p-ARK-1 Antibody (95.Thr 288): AC: Agarose, HRP: Horseradish Peroxidase

The preferred ARK-1 antibody is ARK-1 Antibody (35C1): sc-56881.