p-Akt1 Antibody (5.Ser 473): sc-293125

p-Akt1 Antibody (5.Ser 473) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Ser 473 phosphorylated Akt1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-Akt1 monoclonal antibody (5.Ser 473) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. The Akt family of serine/threonine kinases, which includes Akt1, Akt2, and Akt3, plays a crucial role in cellular signaling pathways that regulate metabolism, growth, and survival. Specifically, Akt1 is known for its involvement in promoting cell survival and growth in response to insulin and growth factors, making Akt1 a key player in metabolic regulation and cancer biology. The phosphorylation of Akt1 at Ser 473 is particularly important as this modification is a marker of activation, which occurs downstream of the phosphatidylinositol 3-kinase (PI3K) pathway. This activation is essential for mediating various cellular processes, including glucose uptake and apoptosis, and is influenced by upstream signals such as insulin and platelet-derived growth factor (PDGF). Understanding the function and regulation of Akt1 is vital, as dysregulation of this pathway is often implicated in metabolic disorders and cancer, highlighting the significance of anti-Akt1 antibody (5.Ser 473) in research aimed at elucidating these critical biological processes.

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p-Akt1 Antibody (5.Ser 473) References:

1. Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site.  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
2. Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization.  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
3. Novel AKT1 mutations associated with cell-cycle abnormalities in gastric carcinoma.  |  Ghatak, S., et al. 2018. Per Med. 15: 79-86. PMID: 29714127
4. AKT1 Mutations in Peripheral Bronchiolar Papilloma: Glandular Papilloma and Mixed Squamous Cell and Glandular Papilloma Is Distinct From Bronchiolar Adenoma.  |  Sasaki, E., et al. 2021. Am J Surg Pathol. 45: 119-126. PMID: 32868527
5. Expression of Akt1 and p-Akt1 in peripheral T cell subsets of multiple sclerosis patients.  |  Oktelik, FB., et al. 2021. Acta Neurol Belg. 121: 1777-1782. PMID: 33034831
6. Effect of AKT1 (p. E17K) Hotspot Mutation on Malignant Tumorigenesis and Prognosis.  |  Chen, Y., et al. 2020. Front Cell Dev Biol. 8: 573599. PMID: 33123537
7. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
8. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase.  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
9. AH/PH domain-mediated interaction between Akt molecules and its potential role in Akt regulation.  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
10. Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA.  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
11. Protein kinase C modulates the insulin-stimulated increase in Akt1 and Akt3 activity in 3T3-L1 adipocytes.  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
12. Akt kinases and 2-deoxyglucose uptake in rat skeletal muscles in vivo: study with insulin and exercise.  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Akt1 Antibody (5.Ser 473) has 129 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Akt1 Antibody (5.Ser 473) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Akt1 Antibody (5.Ser 473) Specifications

Alternate Product Name: Akt1

Product Clone Name: 5.Ser 473

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 473

Epitope Target: Akt1

Epitope Species: human

Product Reactivity/Detection: Ser 473 phosphorylated Akt1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 129 citations in various scientific publications

Available Conjugates/Forms for p-Akt1 Antibody (5.Ser 473): AC: Agarose, HRP: Horseradish Peroxidase

The preferred Akt1 antibody is Akt1 Antibody (B-1): sc-5298.