Akt1 Antibody (B-1): sc-5298

Akt1 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Akt1 of mouse, rat, and human origin by WB, IP, IF, IHCP, FCM, and ELISA. Akt1 monoclonal antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. Akt1, also known as protein kinase B (PKB), plays a crucial role in cellular signaling pathways that regulate cell growth, survival, and metabolism. Akt1 is primarily located in the cytoplasm and is translocated to the plasma membrane upon activation by growth factors such as insulin and platelet-derived growth factor (PDGF). This translocation is essential for activation, as Akt1 interacts with phosphoinositide-dependent kinase-1 (PDK1), which phosphorylates Akt1 at Thr 308, a critical step for full activation. Furthermore, Akt1′s ability to phosphorylate various downstream targets is vital for mediating cellular responses to growth signals, making Akt1 a key player in processes such as glucose metabolism and apoptosis. The phosphorylation of Akt1 at Ser 473, while not required for Thr 308 phosphorylation, further enhances activity and is indicative of promoting cell survival and growth. The regulation of Akt1 is complex and involves various upstream signaling pathways, highlighting importance in both normal physiology and disease states, including cancer. These features make Akt1 monoclonal antibody (B-1) an essential tool for investigating Akt1-mediated signaling pathways and roles in cellular processes and disease research.

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Akt1 Antibody (B-1) References:

1. Identification of a human Akt3 (protein kinase B gamma) which contains the regulatory serine phosphorylation site.  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
2. Mapping of AKT3, encoding a member of the Akt/protein kinase B family, to human and rodent chromosomes by fluorescence in situ hybridization.  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
3. AKT1 provides an essential survival signal required for differentiation and stratification of primary human keratinocytes.  |  Thrash, BR., et al. 2006. J Biol Chem. 281: 12155-62. PMID: 16517604
4. AKT1/BRCA1 in the control of homologous recombination and genetic stability: the missing link between hereditary and sporadic breast cancers.  |  Guirouilh-Barbat, J., et al. 2010. Oncotarget. 1: 691-699. PMID: 21321378
5. AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation.  |  Lu, WC., et al. 2022. FEBS J. 289: 3876-3893. PMID: 35080342
6. Delivery of Active AKT1 to Human Cells.  |  Siddika, T., et al. 2022. Cells. 11: PMID: 36497091
7. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
8. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase.  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
9. AH/PH domain-mediated interaction between Akt molecules and its potential role in Akt regulation.  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
10. Amplification of AKT2 in human pancreatic cells and inhibition of AKT2 expression and tumorigenicity by antisense RNA.  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
11. Protein kinase C modulates the insulin-stimulated increase in Akt1 and Akt3 activity in 3T3-L1 adipocytes.  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
12. Akt kinases and 2-deoxyglucose uptake in rat skeletal muscles in vivo: study with insulin and exercise.  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Akt1 Antibody (B-1) has 728 citations in a variety of scientific publications.

Primary antibodies like Anti-Akt1 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Akt1 Antibody (B-1) Specifications

Alternate Product Name: Akt1

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 345-480

Epitope Target: Akt1

Epitope Species: human

Product Reactivity/Detection: Akt1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 728 citations in various scientific publications

Available Conjugates/Forms for Akt1 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin