Anti-Akt1 Antibody (B-1) recommended for detection of Akt1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-Akt1 Antibody (B-1): sc-5298

Anti-Akt1 Antibody (B-1) is rated 4.6 out of 5 by 85.
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Datasheets

    • Anti-Akt1 Antibody (B-1) is a mouse monoclonal IgG1 κ Akt1 antibody, cited in 636 publications, provided at 200 µg/ml
    • raised against amino acids 345-480 of Akt1 of human origin
    • Anti-Akt1 Antibody (B-1) is recommended for detection of Akt1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-Akt1 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Akt1 (B-1): sc-5298.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Akt1 Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with Akt1 Antibody (B-1) (see ordering information below).
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hello. My name is Ridvan. I am an researcher assistant in Eskisehir Osmangazi University in Eskisehir/Turkey. Can you send me Immunohistochemistry ve Western Blot protocols of Akt-1 (Sc-5298) and p-Akt 1/2/3 antibodies (sc-514032 ). Thank You very much.

Asked by: rido
Thank you for your question. Our recommended protocols for use with both antibodies can be found online. Please contact us for further Technical Support in using these antibodies.
Answered by: Technical Support
Date published: 2019-05-03

is there reactivity for rabbit?

Asked by: exertion7
Thank you for your question. This antibody has been published over 400 times, and we are not aware of any publications in rabbit. Please contact our Technical Service Department if you would like assistance performing sequence analysis using the antibody’s antigen to predict cross reactivity with rabbit.
Answered by: Technical Support
Date published: 2019-04-13

Hi, Where I can check what materials used to wash nitrocellulose or PVDF membrane?

Asked by: Robert1124
Thank you for your question. We recommend washing the membrane with TBS and TBST. The details can be found in our protocol online.
Answered by: Technical Support
Date published: 2019-02-23

Among the different indications of this antibody: Akt1 (B-1): sc-5298, it is its ability to recognize the phosphorylated-AKT1. Since there are different phosphorylation sites, I need to be sure that it applies also for the S473-phosphorylated AKT1.

Asked by: stefania70
Thank you for your question. This antibody can detect the Akt1 protein, both phosphorylated and non-phosphorylated. It is not phospho-specific and does therefore not specifically detect a certain phosphorylation site. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2018-11-30

Dear Whome it may concern, We have some antibodies from other resources that are not work well, and we would like to try a few antibodies. How can we receive free samples? We can send pictures of our not working antibodies if needed.

Asked by: Sevilay Acar
Thank you you for your request. Please contact our European Technical Service team by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com, we would be happy to send you any samples you would like to try.
Answered by: Tech Service
Date published: 2017-10-10

What is the recommended secondary reagent to be used with the Akt1 Antibody (B-1) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-22

Akt1 Antibody (B-1): sc-5298 1) Does it only binds Akt1, or also binds phosphorylated Akt1? 2) How to get a 10 ug sample? How much do we need to pay?

Asked by: tua64317
Thank you for your question. This monoclonal, Akt1 Antibody (B-1): sc-5298, is able to recognize Akt1 and phosphorylated Akt1. This is demonstrated in the Western blot data on our website showing evaluation of inactive and active human recombinant Akt1. Please contact our Technical Service Department to request a complimentary 10 µg sample of this mouse monoclonal antibody for testing. The sample is free of charge. Please call us directly at (800) 457-3801 option 2, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2016-12-21
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Rated 5 out of 5 by from anti-Akt1 (B-1) antibody The anti-Akt1 (B-1) monoclonal antibody worked very well in our Western blotting.
Date published: 2022-06-15
Rated 3 out of 5 by from Medium image I took a not very clear blot with not very intense bands.
Date published: 2021-03-17
Rated 4 out of 5 by from Good Product Antibody works well in western blotting with mouse brain tissue lysate.
Date published: 2019-12-26
Rated 4 out of 5 by from specificità di riconoscimento Ho provato questo anticorpo monoclonale (Free sample) e ha dato risultati soddisfacenti in WB analysis
Date published: 2019-04-26
Rated 5 out of 5 by from Excellent in Western blotting WB of cell lystae of 16-HBE dilution 1:1000 Strong and specific band at the expected molecular weight
Date published: 2019-04-24
Rated 4 out of 5 by from Works on Mouse Liver Used on 40 ug of Mouse Liver Tissue Lysate (cryofractured, then homogenized with Kontes Pellet Pestle using TPer + Complete Protease/Phosphatase Inhib Tablet). Primary 1:300 O/N, Secondary Licor 680LT 1:5000 3 h. Odyssey for imaging.
Date published: 2018-09-18
Rated 4 out of 5 by from AKT I used this antibodies in muscle of Wistar rat in dilution 1:1000. It works very well.
Date published: 2018-07-16
Rated 4 out of 5 by from Good antibodies I got a free sample from company. I used this antibodies in WB in dilution 1:1000. It works very well.
Date published: 2018-06-10
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