p-4E-BP1 Antibody (62.Ser 65): sc-293124

p-4E-BP1 Antibody (62.Ser 65) is a mouse monoclonal IgG2b kappa light chain antibody that detects phosphorylated 4E-BP1 at Ser 65 in human samples, and phosphorylated 4E-BP1 at Ser 64 in mouse and rat samples through western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-4E-BP1 (62.Ser 65) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. 4E-BP1, also known as PHAS-1, plays a crucial role in regulating protein synthesis by binding to eukaryotic translation initiation factor 4E (eIF4E), thereby inhibiting cap-dependent translation. This regulation is vital for cellular responses to growth signals, as phosphorylation of 4E-BP1 by the mTOR pathway releases eIF4E, allowing for translation initiation and protein production. Phosphorylation of 4E-BP1 occurs at multiple sites, with Serine 65 phosphorylation being particularly significant as it marks the transition from a repressive to an active state in translation initiation. Understanding 4E-BP1 phosphorylation dynamics is essential for studying cellular growth and metabolism mechanisms, especially in cancer and metabolic disorders where protein synthesis dysregulation is often observed.

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p-4E-BP1 Antibody (62.Ser 65) References:

1. Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism.  |  Gingras, AC., et al. 1999. Genes Dev. 13: 1422-37. PMID: 10364159
2. Participation of ATM in insulin signalling through phosphorylation of eIF-4E-binding protein 1.  |  Yang, DQ. and Kastan, MB. 2000. Nat Cell Biol. 2: 893-8. PMID: 11146653
3. Phosphorylated 4E-BP1 is associated with poor survival in melanoma.  |  O'Reilly, KE., et al. 2009. Clin Cancer Res. 15: 2872-8. PMID: 19336517
4. Overexpression of phosphorylated 4E-BP1 predicts for tumor recurrence and reduced survival in cervical carcinoma treated with postoperative radiotherapy.  |  Benavente, S., et al. 2009. Int J Radiat Oncol Biol Phys. 75: 1316-22. PMID: 19362787
5. Phosphorylated 4E-BP1 is associated with tumor progression and adverse prognosis in colorectal cancer.  |  Chen, Y., et al. 2017. Neoplasma. 64: 787-794. PMID: 28592131
6. Overexpressed Cyclin D1 and CDK4 proteins are responsible for the resistance to CDK4/6 inhibitor in breast cancer that can be reversed by PI3K/mTOR inhibitors.  |  Cai, Z., et al. 2023. Sci China Life Sci. 66: 94-109. PMID: 35982377
7. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.  |  Pause, A., et al. 1994. Nature. 371: 762-7. PMID: 7935836
8. Identification of phosphorylation sites in the translational regulator, PHAS-I, that are controlled by insulin and rapamycin in rat adipocytes.  |  Fadden, P., et al. 1997. J Biol Chem. 272: 10240-7. PMID: 9092573
9. Phosphorylation of the translational repressor PHAS-I by the mammalian target of rapamycin.  |  Brunn, GJ., et al. 1997. Science. 277: 99-101. PMID: 9204908
10. 4E-BP1, a repressor of mRNA translation, is phosphorylated and inactivated by the Akt(PKB) signaling pathway.  |  Gingras, AC., et al. 1998. Genes Dev. 12: 502-13. PMID: 9472019

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-4E-BP1 Antibody (62.Ser 65) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-p-4E-BP1 Antibody (62.Ser 65) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-4E-BP1 Antibody (62.Ser 65) Specifications

Alternate Product Name: 4EBP1

Product Clone Name: 62.Ser 65

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Ser 65

Epitope Target: 4E-BP1

Epitope Species: human

Product Reactivity/Detection: Ser 65 phosphorylated 4E-BP1 of human origin, and correspondingly phosphorylated Ser 64 4E-BP1 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for p-4E-BP1 Antibody (62.Ser 65): AC: Agarose, HRP: Horseradish Peroxidase

The preferred 4E-BP1 antibody is 4E-BP1 Antibody (P-1): sc-9977.