OTX2 Antibody (D-8): sc-514195

OTX2 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects OTX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OTX2 Antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OTX2 plays a crucial role in early brain development, particularly in the specification of the anterior neural plate and the organization of the primitive streak, which are essential for proper fore-midbrain formation. This transcription factor is vital for the early specification of the neuroectoderm, which ultimately contributes to the development of critical brain structures. Additionally, OTX2 interacts with other transcription factors, such as OTX1, to regulate brain patterning through a dosage-dependent mechanism, ensuring that the isthmic region is correctly positioned and that anterior brain structures are properly formed. The precise regulation of OTX2 levels is essential, as deviations can lead to developmental disorders. The genes encoding OTX1 and OTX2 are located on human chromosomes 2p13 and 14q21-q22, respectively, highlighting their evolutionary conservation and importance in neurodevelopmental processes.

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OTX2 Antibody (D-8) References:

1. Otx genes and the genetic control of brain morphogenesis.  |  Acampora, D., et al. 1999. Mol Cell Neurosci. 13: 1-8. PMID: 10049527
2. Differential transcriptional control as the major molecular event in generating Otx1-/- and Otx2-/- divergent phenotypes.  |  Acampora, D., et al. 1999. Development. 126: 1417-26. PMID: 10068635
3. The TINS Lecture. Understanding the roles of Otx1 and Otx2 in the control of brain morphogenesis.  |  Acampora, D. and Simeone, A. 1999. Trends Neurosci. 22: 116-22. PMID: 10199636
4. Otx1 and Otx2 activities are required for the normal development of the mouse inner ear.  |  Morsli, H., et al. 1999. Development. 126: 2335-43. PMID: 10225993
5. Otx genes in corticogenesis and brain development.  |  Acampora, D., et al. 1999. Cereb Cortex. 9: 533-42. PMID: 10498271
6. Genetic and molecular roles of Otx homeodomain proteins in head development.  |  Acampora, D., et al. 2000. Gene. 246: 23-35. PMID: 10767524
7. Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.  |  Peña, CJ., et al. 2017. Science. 356: 1185-1188. PMID: 28619944
8. MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration.  |  Du, J., et al. 2023. Signal Transduct Target Ther. 8: 351. PMID: 37709738
9. O-GlcNAcylation regulates OTX2's proteostasis.  |  Wulff-Fuentes, E., et al. 2023. iScience. 26: 108184. PMID: 38026167
10. A group 3 medulloblastoma stem cell program is maintained by OTX2-mediated alternative splicing.  |  Saulnier, O., et al. 2024. Nat Cell Biol. 26: 1233-1246. PMID: 39025928
11. Chromosome locations of human EMX and OTX genes.  |  Kastury, K., et al. 1994. Genomics. 22: 41-5. PMID: 7959790
12. Functional equivalency between Otx2 and Otx1 in development of the rostral head.  |  Suda, Y., et al. 1999. Development. 126: 743-57. PMID: 9895322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OTX2 Antibody (D-8) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-OTX2 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OTX2 Antibody (D-8) Specifications

Alternate Product Name: OTX2

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 115-259

Epitope Target: OTX2

Epitope Species: human

Product Reactivity/Detection: OTX2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for OTX2 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin