OSX Antibody (E-6): sc-393060
- OSX Antibody (E-6) is a mouse monoclonal IgG2b κ, cited in 23 publications, provided at 200 µg/ml
- raised against amino acids 172-268 mapping within an internal region of OSX of human origin
- recommended for detection of OSX of mouse, rat and human origin by WB, IP, IF and ELISA
- See OSX (F-3): sc-393325 for OSX antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for OSX Antibody (E-6) for WB applications. These reagents are now offered in bundles with OSX Antibody (E-6) (see ordering information below).
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OSX Antibody (E-6) is a mouse monoclonal IgG2b antibody that detects Osterix (OSX) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OSX is a crucial zinc finger-containing transcriptional activator that plays a vital role in osteoblast differentiation, which is essential for bone formation and maintenance. Anti-OSX antibody (E-6) recognizes OSX predominantly in the nucleus, where OSX binds to GC box promoter elements, thereby activating the transcription of genes necessary for osteoblast maturation. Nuclear localization of OSX enables direct regulation of gene expression, influencing the development of bone-forming cells. In OSX-deficient mice, mesenchymal cells fail to differentiate into osteoblasts, leading to a complete lack of bone matrix deposition and bone formation. OSX expression is linked to Cox-2 activity, suggesting this enzyme may play a role in inducing OSX during skeletal repair processes. OSX monoclonal antibody (E-6) serves as an invaluable tool for researchers studying bone biology and the molecular mechanisms underlying osteoblast differentiation and function.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
OSX Antibody (E-6) References:
- Osx transcriptional regulation is mediated by additional pathways to BMP2/Smad signaling. | Celil, AB., et al. 2005. J Cell Biochem. 95: 518-28. PMID: 15786511
- Methylation of the mouse DIx5 and Osx gene promoters regulates cell type-specific gene expression. | Lee, JY., et al. 2006. Mol Cells. 22: 182-8. PMID: 17085970
- Regulation of osterix (Osx, Sp7) and the Osx promoter by parathyroid hormone in osteoblasts. | Hong, SH., et al. 2009. J Mol Endocrinol. 43: 197-207. PMID: 19505977
- Runx2, osx, and dspp in tooth development. | Chen, S., et al. 2009. J Dent Res. 88: 904-9. PMID: 19783797
- Transcriptional regulation of bone sialoprotein gene expression by Osx. | Yang, Y., et al. 2016. Biochem Biophys Res Commun. 476: 574-579. PMID: 27261434
- LncRNA ODIR1 inhibits osteogenic differentiation of hUC-MSCs through the FBXO25/H2BK120ub/H3K4me3/OSX axis. | He, S., et al. 2019. Cell Death Dis. 10: 947. PMID: 31827076
- Klotho in Osx+-mesenchymal progenitors exerts pro-osteogenic and anti-inflammatory effects during mandibular alveolar bone formation and repair. | Fan, Y., et al. 2022. Signal Transduct Target Ther. 7: 155. PMID: 35538062
- Epigenetic Repression of RUNX2 and OSX Promoters Controls the Nonmineralized State of the Periodontal Ligament. | Gopinathan, G., et al. 2023. Genes (Basel). 14: PMID: 36672941
- TNF-α and OSX mRNA of Salivary Small Extracellular Vesicles in Periodontitis: A Pilot Study. | Han, P., et al. 2023. Tissue Eng Part C Methods. 29: 298-306. PMID: 37358387
- A homozygous SP7/OSX mutation causes osteogenesis and dentinogenesis imperfecta with craniofacial anomalies. | Al-Mutairi, DA., et al. 2024. JBMR Plus. 8: ziae026. PMID: 38562913
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OSX Antibody (E-6) | sc-393060 | 200 µg/ml | $322.00 | |||
OSX Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-535687 | 200 µg Ab; 40 µg BP | $361.00 | |||
OSX Antibody (E-6): m-IgG2b BP-HRP Bundle | sc-549471 | 200 µg Ab; 10 µg BP | $361.00 |