OSX Antibody (F-3): sc-393325

OSX Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects OSX in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-OSX antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OSX, also known as Osterix, is a crucial zinc finger-containing transcriptional activator that plays a vital role in osteoblast differentiation, which is essential for bone formation. OSX is expressed in all developing bones and regulates the expression of genes necessary for osteoblast maturation. Nuclear localization allows OSX to bind to GC box promoter elements, thereby activating mRNA synthesis from target genes. Absence of OSX leads to a failure in the differentiation of periosteal and mesenchymal cells into osteoblasts, resulting in significant impairment in bone matrix deposition and bone formation. Studies have shown that deficiency of cyclooxygenase-2 (Cox-2) correlates with reduced OSX expression, indicating that Cox-2 may play a role in inducing OSX to facilitate skeletal repair. This relationship highlights OSX′s importance not only in bone development but also in potential recovery processes following skeletal injuries.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OSX Antibody (F-3) References:

1. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation.  |  Nakashima, K., et al. 2002. Cell. 108: 17-29. PMID: 11792318
2. Bone morphogenetic protein-2 enhances osterix gene expression in chondrocytes.  |  Yagi, K., et al. 2003. J Cell Biochem. 88: 1077-83. PMID: 12647290
3. BMP-2-induced Osterix expression is mediated by Dlx5 but is independent of Runx2.  |  Lee, MH., et al. 2003. Biochem Biophys Res Commun. 309: 689-94. PMID: 12963046
4. Expression of preosteoblast markers and Cbfa-1 and Osterix gene transcripts in stromal tumour cells of giant cell tumour of bone.  |  Huang, L., et al. 2004. Bone. 34: 393-401. PMID: 15003787
5. Spaciotemporal association and bone morphogenetic protein regulation of sclerostin and osterix expression during embryonic osteogenesis.  |  Ohyama, Y., et al. 2004. Endocrinology. 145: 4685-92. PMID: 15217980
6. Inductive effects of dexamethasone on the gene expression of Cbfa1, Osterix and bone matrix proteins during differentiation of cultured primary rat osteoblasts.  |  Igarashi, M., et al. 2004. J Mol Histol. 35: 3-10. PMID: 15323344
7. BMP-2 and insulin-like growth factor-I mediate Osterix (Osx) expression in human mesenchymal stem cells via the MAPK and protein kinase D signaling pathways.  |  Celil, AB. and Campbell, PG. 2005. J Biol Chem. 280: 31353-9. PMID: 16000303
8. Transcriptional regulation of the osterix (Osx, Sp7) promoter by tumor necrosis factor identifies disparate effects of mitogen-activated protein kinase and NF kappa B pathways.  |  Lu, X., et al. 2006. J Biol Chem. 281: 6297-306. PMID: 16410254
9. p38 mitogen-activated protein kinase regulates osteoblast differentiation through osterix.  |  Wang, X., et al. 2007. Endocrinology. 148: 1629-37. PMID: 17185377
10. The transcriptional factor Osterix directly interacts with RNA helicase A.  |  Amorim, BR., et al. 2007. Biochem Biophys Res Commun. 355: 347-51. PMID: 17303075
11. Regulation of the osterix (Osx, Sp7) promoter by osterix and its inhibition by parathyroid hormone.  |  Barbuto, R. and Mitchell, J. 2013. J Mol Endocrinol. 51: 99-108. PMID: 23682129
12. BMP-2 induced Dspp transcription is mediated by Dlx3/Osx signaling pathway in odontoblasts.  |  Yang, G., et al. 2017. Sci Rep. 7: 10775. PMID: 28883412

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OSX Antibody (F-3) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-OSX Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OSX Antibody (F-3) Specifications

Alternate Product Name: Sp7

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 172-268

Epitope Target: OSX

Epitope Species: human

Product Reactivity/Detection: OSX

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for OSX Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent