ORC6 Antibody (3A4): sc-32735
- ORC6 Antibody (3A4) is a rat monoclonal IgG2a ORC6 antibody, cited in 10 publications, provided at 200 µg/ml
- raised against recombinant ORC6 protein of human origin
- ORC6 Antibody (3A4) is recommended for detection of ORC6 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-ORC6 Antibody (3A4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-32735 X, 200 µg/0.1 ml)
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ORC6 Antibody (3A4). This work is in progress.
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ORC6 Antibody (3A4) is a rat monoclonal IgG2a antibody that detects ORC6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ORC6 antibody (3A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ORC6 is a vital component of the origin recognition complex (ORC), which is essential for DNA replication initiation. ORC6 plays a critical role in forming pre-replication complexes necessary for accurate and timely genome replication. ORC6, along with other ORC subunits, binds to specific origins of replication, serving as a scaffold for additional initiation factors assembly. This binding regulates the cell cycle, particularly during G1-S transition, where cells prepare for DNA synthesis. ORC6 presence in proliferating cell nuclei, including tissues such as testis, underscores its importance in cellular proliferation and genomic stability. ORC6 expression throughout the cell cycle, alongside other ORC proteins, ensures efficient and accurate DNA replication, preventing potential genomic instability that could lead to diseases such as cancer.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ORC6 Antibody (3A4) References:
- Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals. | Natale, DA., et al. 2000. EMBO J. 19: 2728-38. PMID: 10835370
- Identification and characterization of the human ORC6 homolog. | Dhar, SK. and Dutta, A. 2000. J Biol Chem. 275: 34983-8. PMID: 10945994
- Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins. | Thome, KC., et al. 2000. J Biol Chem. 275: 35233-41. PMID: 10954718
- Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis. | Méndez, J. and Stillman, B. 2000. Mol Cell Biol. 20: 8602-12. PMID: 11046155
- The human origin recognition complex protein 1 dissociates from chromatin during S phase in HeLa cells. | Kreitz, S., et al. 2001. J Biol Chem. 276: 6337-42. PMID: 11102449
- Assembly of the human origin recognition complex. | Vashee, S., et al. 2001. J Biol Chem. 276: 26666-73. PMID: 11323433
- Architecture of the human origin recognition complex. | Dhar, SK., et al. 2001. J Biol Chem. 276: 29067-71. PMID: 11395502
- Identification of HsORC4, a member of the human origin of replication recognition complex. | Quintana, DG., et al. 1997. J Biol Chem. 272: 28247-51. PMID: 9353276
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORC6 Antibody (3A4) | sc-32735 | 200 µg/ml | $322.00 | |||
ORC6 Antibody (3A4) X | sc-32735 X | 200 µg/0.1 ml | $322.00 | |||
ORC6 Antibody (3A4) AC | sc-32735 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ORC6 Antibody (3A4) HRP | sc-32735 HRP | 200 µg/ml | $322.00 | |||
ORC6 Antibody (3A4) FITC | sc-32735 FITC | 200 µg/ml | $336.00 | |||
ORC6 Antibody (3A4) PE | sc-32735 PE | 200 µg/ml | $349.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 488 | sc-32735 AF488 | 200 µg/ml | $364.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 546 | sc-32735 AF546 | 200 µg/ml | $364.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 594 | sc-32735 AF594 | 200 µg/ml | $364.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 647 | sc-32735 AF647 | 200 µg/ml | $364.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 680 | sc-32735 AF680 | 200 µg/ml | $364.00 | |||
ORC6 Antibody (3A4) Alexa Fluor® 790 | sc-32735 AF790 | 200 µg/ml | $364.00 |