ORC6 Activators
Chemical activators of ORC6 include a variety of compounds that influence the protein's activity within the cellular context of DNA replication initiation. ATP is fundamental for ORC6 activity as it provides the necessary energy for phosphorylation processes, with kinases catalyzing the addition of phosphate groups to ORC6, which is a critical step for the initiation of DNA replication.
Furthermore, certain DNA replication inhibitors like Aphidicolin and Thymidine indirectly activate ORC6 by creating a cellular environment that requires the re-initiation of DNA replication, thus necessitating the ORC complex's activity. Similarly, Hydroxyurea triggers the cell's checkpoint pathways by depleting dNTPs, leading to a situation where ORC6 activation is part of the cellular response to ensure the fidelity of replication restart. Mimosine's action in arresting cells at the late G1 phase elevates activities of proteins like ORC6 that are crucial for initiating DNA replication. Additionally, Topoisomerase inhibitors, such as Topotecan, induce DNA damage that can lead to the activation of ORC6 within the context of the DNA damage response, as the protein is a component of the ORC complex that is vital for the integrity of replication initiation.
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
ADP | 58-64-0 | sc-507362 | 5 g | $54.00 | ||
ATP can provide the energy necessary for the phosphorylation reactions catalyzed by kinases. Since ORC6 is a part of the origin recognition complex (ORC) involved in DNA replication initiation, ATP may be required for the activation of ORC6 through phosphorylation by kinases which are needed for ORC assembly and function. | ||||||
Thymidine | 50-89-5 | sc-296542 sc-296542A sc-296542C sc-296542D sc-296542E sc-296542B | 1 g 5 g 100 g 250 g 1 kg 25 g | $49.00 $73.00 $270.00 $458.00 $1758.00 $114.00 | 16 | |
High concentrations of thymidine can lead to DNA synthesis arrest, which indirectly may result in increased activity of proteins involved in the initiation of DNA replication, including ORC6, as the cell tries to overcome the thymidine block and resume replication. | ||||||
L-Mimosine | 500-44-7 | sc-201536A sc-201536B sc-201536 sc-201536C | 25 mg 100 mg 500 mg 1 g | $36.00 $88.00 $220.00 $436.00 | 8 | |
Mimosine blocks cells at the late G1 phase by inhibiting DNA replication. This arrest can lead to the activation of pathways that regulate the initiation of DNA replication, potentially increasing the activity of ORC6 as the cell prepares for the eventual progression into the S phase. | ||||||
Topotecan | 123948-87-8 | sc-338718 | 100 mg | $582.00 | ||
Topoisomerase inhibitors such as Topotecan create DNA damage by stabilizing topoisomerase-DNA cleavage complexes, which can activate the DNA damage response and replication checkpoints. This may indirectly lead to the functional activation of ORC6 as part of the cellular effort to maintain replication integrity, which involves the ORC complex. | ||||||