ORC1 Antibody (3H2792): sc-71752

ORC1 Antibody (3H2792) is a mouse monoclonal IgG1 antibody that detects ORC1 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). ORC1, a critical component of the origin recognition complex, plays a vital role in the initiation of DNA replication by binding to specific origins of replication, thereby facilitating the assembly of additional initiation factors necessary for the formation of pre-replication complexes. This process is essential for ensuring accurate DNA replication and cell division, which are fundamental for maintaining genomic stability. ORC1 is predominantly located in the nucleus of proliferating cells, including tissues such as the testis, where ORC1 is expressed alongside other ORC subunits. Notably, while ORC1 and ORC2 are present at similar levels throughout the cell cycle, ORC2 remains stably associated with chromatin, highlighting ORC2′s importance in the regulation of DNA replication. ORC1′s interaction with other ORC proteins, particularly in forming a core complex with ORC2, ORC3, ORC4, and ORC5, underscores ORC1′s critical function in the G1-S transition of the cell cycle, making ORC1 a key player in cellular proliferation and a valuable target for research in cell biology and cancer studies.

The preferred ORC1 antibody is ORC1 Antibody (7A7): sc-23887.