ORC1 Antibody (3C166): sc-71751

ORC1 Antibody (3C166) is a mouse monoclonal IgG1 antibody that detects ORC1 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). ORC1, a critical component of the origin recognition complex, plays a vital role in the initiation of DNA replication by binding to specific origins of replication, thereby facilitating the assembly of additional initiation factors necessary for the formation of pre-replication complexes. This process is essential for ensuring accurate DNA replication and cell division, which are fundamental for maintaining genomic stability. ORC1 is predominantly located in the nucleus of proliferating cells, including tissues such as the testis, where ORC1 is expressed alongside other ORC subunits. Notably, ORC1 and ORC2 maintain equivalent concentrations throughout the cell cycle, but only ORC2 remains stably bound to chromatin, highlighting the dynamic nature of these proteins during the G1-S transition. The interaction of ORC1 with other ORC subunits, such as ORC2, ORC3, ORC4, and ORC5, to form a core complex underscores ORC1′s importance in orchestrating the complex events of DNA replication initiation. Anti-ORC1 antibody (3C166) serves as an invaluable tool for researchers studying the mechanisms of DNA replication and the regulation of cell cycle progression.

The preferred ORC1 antibody is ORC1 Antibody (7A7): sc-23887.