Optineurin Antibody (C-2): sc-166576
- Optineurin Antibody (C-2) is a mouse monoclonal IgG2b κ Optineurin antibody, cited in 37 publications, provided at 200 µg/ml
- raised against a peptide mapping near the N-terminus of Optineurin of human origin
- Optineurin Antibody (C-2) is recommended for detection of Optineurin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine
- Anti-Optineurin Antibody (C-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Optineurin Antibody (C-2) for WB and IHC(P) applications. These reagents are now offered in bundles with Optineurin Antibody (C-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
Optineurin Antibody (C-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Optineurin (OPTN) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-OPTN antibody (C-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OPTN (C-2) antibody recognizes a protein also known as FIP2, E3-14.7K-interacting protein, and NEMO-related protein, which plays a crucial role in cellular processes such as cell morphogenesis, membrane trafficking, and transcriptional activation through interactions with key proteins like Rab8 and Huntingtin. OPTN monoclonal antibody (C-2) targets a protein involved in regulating apoptosis and inflammation, potentially utilizing pathways associated with tumor necrosis factor alpha or FAS-ligand. Mutations in OPTN have been linked to normal-tension glaucoma and adult-onset primary open-angle glaucoma, highlighting significance in ocular health. Structurally, OPTN is a 617 amino acid protein characterized by leucine zippers and leucine-rich regions, as well as a potential Cys2-His-Cys zinc finger motif located between residues 553 and 582, which may play a role in functional interactions. OPTN is primarily localized to the Golgi apparatus, with expression in various human tissues, including trabecular meshwork, retina, brain, and liver, indicating diverse biological roles.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Optineurin Antibody (C-2) References:
- Molecular cloning, genomic structure, and protein characterization of mouse optineurin. | Rezaie, T. and Sarfarazi, M. 2005. Genomics. 85: 131-8. PMID: 15607428
- Optineurin links myosin VI to the Golgi complex and is involved in Golgi organization and exocytosis. | Sahlender, DA., et al. 2005. J Cell Biol. 169: 285-95. PMID: 15837803
- Molecular cloning and expression profiling of optineurin in the rhesus monkey. | Rezaie, T., et al. 2005. Invest Ophthalmol Vis Sci. 46: 2404-10. PMID: 15980228
- Analysis of rare variants and common haplotypes in the optineurin gene in Swedish glaucoma cases. | Jansson, M., et al. 2005. Ophthalmic Genet. 26: 85-9. PMID: 16020311
- Clinical features and course of patients with glaucoma with the E50K mutation in the optineurin gene. | Aung, T., et al. 2005. Invest Ophthalmol Vis Sci. 46: 2816-22. PMID: 16043855
- Optineurin increases cell survival and translocates to the nucleus in a Rab8-dependent manner upon an apoptotic stimulus. | De Marco, N., et al. 2006. J Biol Chem. 281: 16147-56. PMID: 16569640
- [Polymorphisms of myocilin and optineurin in primary open angle glaucoma patients]. | Yao, HY., et al. 2006. Zhonghua Yi Xue Za Zhi. 86: 554-9. PMID: 16681888
- [Low penetrance of His26Asp mutation in the optineurin gene in a Japanese family with normal-tension glaucoma]. | Yasuda, N., et al. 2006. Nippon Ganka Gakkai Zasshi. 110: 594-600. PMID: 16972651
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Optineurin Antibody (C-2) | sc-166576 | 200 µg/ml | $322.00 | |||
Optineurin Antibody (C-2): m-IgGκ BP-HRP Bundle | sc-521662 | 200 µg Ab, 40 µg BP | $361.00 | |||
Optineurin Antibody (C-2): m-IgG2b BP-HRP Bundle | sc-548949 | 200 µg Ab; 10 µg BP | $361.00 | |||
Optineurin Antibody (C-2) AC | sc-166576 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Optineurin Antibody (C-2) HRP | sc-166576 HRP | 200 µg/ml | $322.00 | |||
Optineurin Antibody (C-2) FITC | sc-166576 FITC | 200 µg/ml | $336.00 | |||
Optineurin Antibody (C-2) PE | sc-166576 PE | 200 µg/ml | $349.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 488 | sc-166576 AF488 | 200 µg/ml | $364.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 546 | sc-166576 AF546 | 200 µg/ml | $364.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 594 | sc-166576 AF594 | 200 µg/ml | $364.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 647 | sc-166576 AF647 | 200 µg/ml | $364.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 680 | sc-166576 AF680 | 200 µg/ml | $364.00 | |||
Optineurin Antibody (C-2) Alexa Fluor® 790 | sc-166576 AF790 | 200 µg/ml | $364.00 | |||
Optineurin (C-2) Neutralizing Peptide | sc-166576 P | 100 µg/0.5 ml | $69.00 |