Opalin Antibody (H-12): sc-374490
- Opalin Antibody (H-12) is a mouse monoclonal IgG1 κ Opalin antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-143 representing full length Opalin of mouse origin
- Opalin Antibody (H-12) is recommended for detection of Opalin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Opalin Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Opalin Antibody (H-12) for WB applications. These reagents are now offered in bundles with Opalin Antibody (H-12) (see ordering information below).
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Opalin Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Opalin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Opalin (H-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Opalin, also known as HTMP10, oligodendrocytic myelin paranodal and inner loop protein, or transmembrane protein 10 (TMEM10), is a 141 amino acid single-pass type I membrane protein that plays a crucial role in the central nervous system by being specifically expressed in oligodendrocytes, the cells responsible for myelinating axons in the brain. The localization of Opalin in oligodendrocytes is vital for maintaining the integrity of myelin sheaths, which are essential for efficient nerve signal transmission. Disruption of Opalin function can lead to demyelination and is implicated in various neurological disorders, highlighting Opalin′s importance in both normal brain function and disease pathology. The gene encoding Opalin is located on human chromosome 10q24.1, a region that spans nearly 135 million base pairs and encodes approximately 1,200 genes, some of which are associated with conditions such as Charcot-Marie-Tooth disease and multiple endocrine neoplasia type 2.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Opalin Antibody (H-12) References:
- Identification and characterization of a novel human brain-specific gene, homologous to S. scrofa tmp83.5, in the chromosome 10q24 critical region for temporal lobe epilepsy and spastic paraplegia. | Nobile, C., et al. 2002. Gene. 282: 87-94. PMID: 11814680
- Molecular cell biology of Charcot-Marie-Tooth disease. | Berger, P., et al. 2002. Neurogenetics. 4: 1-15. PMID: 12030326
- An oligodendrocyte enhancer in a phylogenetically conserved intron region of the mammalian myelin gene Opalin. | Aruga, J., et al. 2007. J Neurochem. 102: 1533-1547. PMID: 17442045
- Cowden syndrome-affected patients with PTEN promoter mutations demonstrate abnormal protein translation. | Teresi, RE., et al. 2007. Am J Hum Genet. 81: 756-67. PMID: 17847000
- Opalin, a transmembrane sialylglycoprotein located in the central nervous system myelin paranodal loop membrane. | Yoshikawa, F., et al. 2008. J Biol Chem. 283: 20830-40. PMID: 18490449
- PTEN: a new guardian of the genome. | Yin, Y. and Shen, WH. 2008. Oncogene. 27: 5443-53. PMID: 18794879
- Jackson-Weiss and Crouzon syndromes are allelic with mutations in fibroblast growth factor receptor 2. | Jabs, EW., et al. 1994. Nat Genet. 8: 275-9. PMID: 7874170
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Opalin Antibody (H-12) | sc-374490 | 200 µg/ml | $316.00 | |||
Opalin Antibody (H-12): m-IgG Fc BP-HRP Bundle | sc-529756 | 200 µg Ab; 10 µg BP | $354.00 | |||
Opalin Antibody (H-12): m-IgGκ BP-HRP Bundle | sc-522756 | 200 µg Ab, 40 µg BP | $354.00 | |||
Opalin Antibody (H-12): m-IgG1 BP-HRP Bundle | sc-543673 | 200 µg Ab; 20 µg BP | $354.00 | |||
Opalin Antibody (H-12) AC | sc-374490 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Opalin Antibody (H-12) HRP | sc-374490 HRP | 200 µg/ml | $316.00 | |||
Opalin Antibody (H-12) FITC | sc-374490 FITC | 200 µg/ml | $330.00 | |||
Opalin Antibody (H-12) PE | sc-374490 PE | 200 µg/ml | $343.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 488 | sc-374490 AF488 | 200 µg/ml | $357.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 546 | sc-374490 AF546 | 200 µg/ml | $357.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 594 | sc-374490 AF594 | 200 µg/ml | $357.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 647 | sc-374490 AF647 | 200 µg/ml | $357.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 680 | sc-374490 AF680 | 200 µg/ml | $357.00 | |||
Opalin Antibody (H-12) Alexa Fluor® 790 | sc-374490 AF790 | 200 µg/ml | $357.00 |