Date published: 2025-10-12

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Opalin Antibody (E-8): sc-376128

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Datasheets
  • Opalin Antibody (E-8) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
  • raised against amino acids 1-143 representing full length Opalin of mouse origin
  • Anti-Opalin Antibody (E-8) is recommended for detection of Opalin of mouse and rat origin by WB, IP, IF and ELISA
  • Anti-Opalin Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for Opalin Antibody (E-8) for WB applications. This reagent is now offered in a bundle with Opalin Antibody (E-8) (see ordering information below).

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    Opalin Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects Opalin in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Opalin (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Opalin, also referred to as HTMP10 or transmembrane protein 10 (TMEM10), is a 141 amino acid single-pass type I membrane protein predominantly expressed in oligodendrocytes within the brain. This protein plays a crucial role in the formation and maintenance of myelin sheaths, which are essential for proper conduction of electrical impulses along nerve fibers. The integrity of myelin is vital for efficient functioning of the nervous system, and any disruption in myelin formation can lead to severe neurological disorders, including multiple sclerosis. The gene encoding Opalin is located on human chromosome 10q24.1, a region that spans nearly 135 million base pairs and encodes approximately 1,200 genes, some of which are implicated in various genetic disorders. By utilizing anti-Opalin antibody (E-8), researchers can gain insights into Opalin′s role in myelination and potential implications in neurodegenerative diseases.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Opalin Antibody (E-8)

    sc-376128
    200 µg/ml
    $316.00

    Opalin Antibody (E-8): m-IgG2b BP-HRP Bundle

    sc-548752
    200 µg Ab; 10 µg BP
    $354.00

    Opalin Antibody (E-8) AC

    sc-376128 AC
    500 µg/ml, 25% agarose
    $416.00

    Opalin Antibody (E-8) HRP

    sc-376128 HRP
    200 µg/ml
    $316.00

    Opalin Antibody (E-8) FITC

    sc-376128 FITC
    200 µg/ml
    $330.00

    Opalin Antibody (E-8) PE

    sc-376128 PE
    200 µg/ml
    $343.00

    Opalin Antibody (E-8) Alexa Fluor® 488

    sc-376128 AF488
    200 µg/ml
    $357.00

    Opalin Antibody (E-8) Alexa Fluor® 546

    sc-376128 AF546
    200 µg/ml
    $357.00

    Opalin Antibody (E-8) Alexa Fluor® 594

    sc-376128 AF594
    200 µg/ml
    $357.00

    Opalin Antibody (E-8) Alexa Fluor® 647

    sc-376128 AF647
    200 µg/ml
    $357.00

    Opalin Antibody (E-8) Alexa Fluor® 680

    sc-376128 AF680
    200 µg/ml
    $357.00

    Opalin Antibody (E-8) Alexa Fluor® 790

    sc-376128 AF790
    200 µg/ml
    $357.00

    What is the recommended starting dilution for Western blot for sc-376128: Opalin (E-8) monoclonal antibody?

    Asked by: jenniferc
    The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
    Answered by: Technical Support
    Date published: 2017-03-22
    • y_2025, m_10, d_9, h_7CST
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    Rated 5 out of 5 by from Works as expectedUsed it at 1:200 dilution in a WB in in mouse brain tissue extract.
    Date published: 2017-01-20
    Rated 5 out of 5 by from Good IHC staining at 1:150 dilutionGood IHC staining at 1:150 dilution.
    Date published: 2015-06-22
    Rated 5 out of 5 by from Produced positive Western Blot data of glycosylatedProduced positive Western Blot data of glycosylated Opalin expression in rat cerebellum tissue extract. -SCBT QC
    Date published: 2015-04-12
    Rated 5 out of 5 by from Great Western Blot data of Opalin expressionGreat Western Blot data of Opalin expression in mouse brain tissue extract. -SCBT QC
    Date published: 2014-07-09
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    Opalin Antibody (E-8) is rated 5.0 out of 5 by 4.
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