Anti-Oct-1 Antibody (12F11) is a mouse monoclonal IgG1 κ Oct-1 antibody, cited in 41 publications, provided at 200 µg/ml
raised against amino acids 462-760 of Oct-1 of Xenopus laevis origin
Anti-Oct-1 Antibody (12F11) is recommended for detection of Oct-1 of mouse, rat, human and Xenopus laevis origin by WB, IP, IF and IHC(P)
Anti-Oct-1 Antibody (12F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-8024 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oct-1 (12F11): sc-8024.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Oct-1 Antibody (12F11) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-1 Antibody (12F11) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Oct-1 Antibody (12F11) is a high quality monoclonal Oct-1 antibody (also designated POU class 2 homeobox 1 antibody, OCT1 antibody, OTF1 antibody, or oct-1B antibody) suitable for the detection of the Oct-1 protein of mouse, rat, human and Xenopus laevis origin. Oct-1 Antibody (12F11) is available as both the non-conjugated anti-Oct-1 antibody form, as well as multiple conjugated forms of anti-Oct-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. POU domain proteins contain a bipartite DNA binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. The POU dimer from the Oct-1 gene formed on the palindromic Oct factor-recognition element (PORE), which is comprised of an inverted pair of homeodomain-binding sites separated by exactly 5 bp (ATTTGAAATGCAAAT), could recruit the transcriptional co-activator OBF1. Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, ATTTGCAT, and the proteins that bind to it. This is a regulatory element important for tissue- and cell-specific transcription, as well as for transcription of a number of housekeeping genes. The Oct-1 gene encodes one protein, NF-A1, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, which is encoded by the Oct-2 gene.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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