Occludin Antibody (E-5): sc-133256

Occludin Antibody (E-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Occludin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Occludin antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Occludin is an integral membrane protein that plays a crucial role in forming and maintaining tight junctions in epithelial and endothelial cells, which are essential for regulating paracellular permeability and maintaining blood-brain barrier integrity. Occludin features a tetraspan structure with two extracellular loops that facilitate interactions with other tight junction proteins, contributing to the overall architecture and function of the tight junction complex. Post-translational modifications, including phosphorylation, are vital for Occludin localization and functional regulation. Phosphorylation of Occludin can influence tight junction formation, impacting cellular permeability to large molecules. Polyunsaturated fatty acids up-regulate Occludin expression, which enhances transendothelial cell resistance, further highlighting Occludin′s importance in maintaining cellular barriers. Occludin shows high expression at cell-cell contact sites in brain tissue, while non-neural tissues display lower expression levels and a more discontinuous distribution. Changes in Occludin expression may enhance paracellular permeability and could be linked to tight junction damage in various pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Occludin Antibody (E-5) References:

1. Occludin degradation makes brain microvascular endothelial cells more vulnerable to reperfusion injury in vitro.  |  Zhang, Y., et al. 2021. J Neurochem. 156: 352-366. PMID: 32531803
2. Occludin Promotes Adhesion of CD8+ T Cells and Melanocytes in Vitiligo via the HIF-1α Signaling Pathway.  |  Zou, P., et al. 2022. Oxid Med Cell Longev. 2022: 6732972. PMID: 35222802
3. Higher serum occludin after successful reperfusion Is associated with early neurological deterioration.  |  Li, W., et al. 2022. CNS Neurosci Ther. 28: 999-1007. PMID: 35338575
4. Cleavage of Occludin by Cigarette Smoke-Elicited Cathepsin S Increases Permeability of Lung Epithelial Cells.  |  Bigot, P., et al. 2022. Antioxidants (Basel). 12: PMID: 36670867
5. The tight junction protein occludin modulates blood-brain barrier integrity and neurological function after ischemic stroke in mice.  |  Sugiyama, S., et al. 2023. Sci Rep. 13: 2892. PMID: 36806348
6. Roles of epidermal growth factor receptor, claudin-1 and occludin in multi-step entry of hepatitis C virus into polarized hepatoma spheroids.  |  So, CW., et al. 2023. PLoS Pathog. 19: e1011887. PMID: 38157366
7. Occludin: a novel integral membrane protein localizing at tight junctions.  |  Furuse, M., et al. 1993. J Cell Biol. 123: 1777-88. PMID: 8276896
8. Molecular dissection of tight junctions.  |  Tsukita, S., et al. 1996. Cell Struct Funct. 21: 381-5. PMID: 9118244
9. Possible involvement of phosphorylation of occludin in tight junction formation.  |  Sakakibara, A., et al. 1997. J Cell Biol. 137: 1393-401. PMID: 9182670
10. Occludin as a possible determinant of tight junction permeability in endothelial cells.  |  Hirase, T., et al. 1997. J Cell Sci. 110 (Pt 14): 1603-13. PMID: 9247194
11. Phosphorylation of occludin correlates with occludin localization and function at the tight junction.  |  Wong, V. 1997. Am J Physiol. 273: C1859-67. PMID: 9435490
12. Regulation of tight junction permeability and occludin expression by polyunsaturated fatty acids.  |  Jiang, WG., et al. 1998. Biochem Biophys Res Commun. 244: 414-20. PMID: 9514943

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Occludin Antibody (E-5) has 161 citations in a variety of scientific publications.

Primary antibodies like Anti-Occludin Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Occludin Antibody (E-5) Specifications

Alternate Product Name: Occludin

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 132-411

Epitope Target: Occludin

Epitope Species: human

Product Reactivity/Detection: Occludin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 161 citations in various scientific publications

Available Conjugates/Forms for Occludin Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Occludin antibody is Occludin Antibody (F-7): sc-271842.