Anti-Ob Antibody (H-5) recommended for detection of precursor and mature Ob of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Ob Antibody (H-5): sc-393043

Anti-Ob Antibody (H-5) is rated 2.0 out of 5 by 4.
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Datasheets

    • Ob Antibody (H-5) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 16-40 at the N-terminus of Ob of human origin
    • Anti-Ob Antibody (H-5) is recommended for detection of precursor and mature Ob of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Ob Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ob (H-5): sc-393043.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ob Antibody (H-5) for WB applications. These reagents are now offered in bundles with Ob Antibody (H-5) (see ordering information below).
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What application is the blocking peptide sc-393043 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from No specific band in adipose tissue by WB I tried this antibody in both epididymal and subcutaneous adipose tissue using western blot, but only get a band at ~25kDa, no band was found at~16 kDa.
Date published: 2021-03-25
Rated 1 out of 5 by from Failed I failed to try to detect leptin by Western blot of 3 ug of human adipose tissue.
Date published: 2018-05-06
Rated 1 out of 5 by from no results I received this antibody as a sample. I tried it on a western blot and there was no results. I will try it again and update when it happens
Date published: 2018-03-02
Rated 5 out of 5 by from Good Western blot data of human recombinant Ob Good Western blot data of human recombinant Ob. -SCBT QC
Date published: 2014-05-20
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