Ob Antibody (H-5): sc-393043

Ob Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ob (leptin) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Ob antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Leptin, the product of the Ob gene, plays a crucial role in regulating energy balance by inhibiting hunger, which in turn helps to manage body weight. Leptin is primarily secreted by adipose tissue and acts on the hypothalamus to signal satiety, making leptin a key player in the complex network of appetite regulation and energy homeostasis. The significance of leptin extends beyond mere appetite control; leptin also influences various physiological processes, including metabolism and immune function. Notably, mutations in the Ob gene can lead to severe obesity and metabolic disorders, underscoring the importance of leptin in maintaining healthy body weight and metabolic health. The cloning and sequence analysis of the mouse Ob gene and its human counterpart have revealed a highly conserved 167 amino acid open reading frame, with an amino acid sequence that is 84% identical between species, highlighting its evolutionary significance. A specific nonsense mutation in codon 105 has been identified in the original congenic C57BL/6J Ob/Ob mouse strain, which serves as a valuable model for studying obesity and related metabolic diseases.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ob Antibody (H-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Ob Antibody (H-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ob Antibody (H-5) Specifications

Alternate Product Name: Leptin

Product Clone Name: H-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 16-40

Epitope Target: Ob

Epitope Species: human

Product Reactivity/Detection: precursor and mature Ob

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Ob Antibody (H-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Ob antibody is Ob Antibody (F-3): sc-48408.